. Advances in marine environmental research : proceedings of a symposium. Marine ecology -- Research; Marine ecology. sic:^c Figure 10-2. Diagram of Stimulation-Recording Chamber ("Olfactometer"). NOTE: Stimulus is injected with a syringe (A) into a seawater flow (B) over the dissected lobster antennule. The antennule is perfused through a micro- pipette with oxygenated lobster saline (C) which exits the antennule into a bath of saline (D). To make recordings, one small bundle of nerve fibers is lifted from the saline bath into the air with a platinum hook electrode (E). Seawater and
. Advances in marine environmental research : proceedings of a symposium. Marine ecology -- Research; Marine ecology. sic:^c Figure 10-2. Diagram of Stimulation-Recording Chamber ("Olfactometer"). NOTE: Stimulus is injected with a syringe (A) into a seawater flow (B) over the dissected lobster antennule. The antennule is perfused through a micro- pipette with oxygenated lobster saline (C) which exits the antennule into a bath of saline (D). To make recordings, one small bundle of nerve fibers is lifted from the saline bath into the air with a platinum hook electrode (E). Seawater and saline baths are separated by a Sylgard cork, through which the antennule passes. Neurophysiology Electrophysiological data were obtained from chemoreceptors of oil-exposed and normal lobsters, some of which had been observed behaviorally. This permits a comparison between the neural chemosensory input the animal received, and the resultant behavior after processing through higher nerve centers. Such a comparison is a necessary step in determining whether oil interferes with behavior through chemoreception. To measure neurophysiological activity, the lateral flagellum of the antennule of a lobster was removed and placed in fresh seawater. The cut proximal end was inserted through a Sylgard cork. Three to four cuticular rings were removed. The distal tip was cut and the antennule placed in a lobster sahne bath in the stimulation-recording chamber (Figure 10-2). A micropipette was inserted snugly into the distal tip, and perfusion with oxygenated lobster 126. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Environmental Research Laboratory (Narragansett, R. I. ); Jacoff, Francine Sakin. Narragansett, R. I. : Environmental Research Laboratory, Office of Research and Development, U. S. Environmental Protection
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