. Contributions from the Botanical Laboratory and the Morris Arboretum of the University of Pennsylvania, vol. 14. Botany; Botany. 1933 1934 1935 1936. Year 1982 25 24 25 Zf, 27 28 29 30 * ^ ^r' ^' o^^^^^^ occurrence of new forks in plantings of red pine. A. Distribution of forks on 852 trees, 6-7 years old, from five plantations. B. Distribution of forks on 100 trees from a plantation in which the trees averaged 23 years old. HISTOLOGICAL STUDIES Material for histological examination was fixed in a chromic acid—forma- lin—salcylic acid solution (2), dehydrated with the N-butyl alcohol technic
. Contributions from the Botanical Laboratory and the Morris Arboretum of the University of Pennsylvania, vol. 14. Botany; Botany. 1933 1934 1935 1936. Year 1982 25 24 25 Zf, 27 28 29 30 * ^ ^r' ^' o^^^^^^ occurrence of new forks in plantings of red pine. A. Distribution of forks on 852 trees, 6-7 years old, from five plantations. B. Distribution of forks on 100 trees from a plantation in which the trees averaged 23 years old. HISTOLOGICAL STUDIES Material for histological examination was fixed in a chromic acid—forma- lin—salcylic acid solution (2), dehydrated with the N-butyl alcohol technic, and cut on a sliding microtome in sections varying from 5 to 15 |j in thick- ness. It was found necessary in working with the older tissues to extend the period of dehydration and infiltration to 10 days or 2 weeks in order to insure complete penetration by the alcohol and paraffin. Buds, precocious shoots, wood from the older portions of the trunk, and roots were examined for abnormal anatomical features and for the presence of fungus hyphae. The most successful stain employed was Cartwright's picro-analine blue combi- nation (1), although the chromatic hyphae that appeared in the old wood. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original University of Pennsylvania. Botanical Laboratory; University of Pennsylvania. Morris Arboretum. Philadelphia : [s. n. ]
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