. Pathology and bacteriology [electronic resource]. Ophthalmology; Eye; Eye; Bacteriology; Ophthalmology; Eye; Bacteriology; Eye. 696 APPENDIX ON LABORATORY METHODS Cutting.—Apart from good embedding success in getting good sections depends on having a sharp knife. For this reason it is always desirable to cut off the part of the specimen which is not required with an old blade, reserving the use of the sharp blade for the part from which it is desired to obtain good sections. The best form of microtome for paraffin and celloidin is the base sledge (Leitz) (Figs. 300, 301). For paraffin alone
. Pathology and bacteriology [electronic resource]. Ophthalmology; Eye; Eye; Bacteriology; Ophthalmology; Eye; Bacteriology; Eye. 696 APPENDIX ON LABORATORY METHODS Cutting.—Apart from good embedding success in getting good sections depends on having a sharp knife. For this reason it is always desirable to cut off the part of the specimen which is not required with an old blade, reserving the use of the sharp blade for the part from which it is desired to obtain good sections. The best form of microtome for paraffin and celloidin is the base sledge (Leitz) (Figs. 300, 301). For paraffin alone Minot's or the Cambridge rocker are excellent, whilst for celloidin the Jung microtome is good. In cutting celloidin the knife should be set at an obUque angle to the specimen, and should be kept continuously wet with methylated Fig. 301. Leitz "base sledge" microtome in use for cutting paraffin sections. In the base sledge model this is performed by means of a dripper. As the sections are cut they should be placed in 60 per cent, alcohol by means of a camel-hair brush, and if it is desired to mount them in series a long dish or glass pen tray should be used in which to place them in order. In making serial sections by this method it is desirable to mount each one separately, but if it is not required to examine them under the high powers of the microscope, as in tracing the degeneration of nerve fibres, they may be mounted in the following manner: A large glass plate is smeared with a solution of dextrin; over this is poured a solution of photoxylin in equal parts of ether and alcohol of about the consistency of treacle. When setting, the celloidin sections should be arranged in order on the plate. After it is set the whole is plunged into methylated spirit; the film containing the sections can then be readily stripped from the glass and the sections treated in the ordinary Please note that these images are extracted from scanned page images that may
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