. Biological structure and function; proceedings. Biochemistry; Cytology. 76 J. B. CHAPPELL pathway. The possibiHty must not be overlooked that glutamate dehydro- genase serves a synthetic rather than a degradative function in hver mito- chondria. The phosphate requirement for DPN-stimulated glutamate oxidation [8, 9] and, when oligomycin [10] is present, the requirement for ADP [11] are presumably reflections of the demands of the substrate level phos- phorylation associated with a-ketoglutarate oxidation. These requirements have also been observed when ferricyanide acted as terminal electron
. Biological structure and function; proceedings. Biochemistry; Cytology. 76 J. B. CHAPPELL pathway. The possibiHty must not be overlooked that glutamate dehydro- genase serves a synthetic rather than a degradative function in hver mito- chondria. The phosphate requirement for DPN-stimulated glutamate oxidation [8, 9] and, when oligomycin [10] is present, the requirement for ADP [11] are presumably reflections of the demands of the substrate level phos- phorylation associated with a-ketoglutarate oxidation. These requirements have also been observed when ferricyanide acted as terminal electron acceptor. 100 r 90 80 S 70 U -S 60 o I 50 40 30 20 Lewisite. 0 12 3 4 5 Time (min) Fig. 6. The effect of 2 :3-dimercaptopropanol (BAL) on the inhibition of glutamate oxidation by ^-chlorovinylarsenious oxide. /3-chlorovinylarsenious oxide, 0"5 Mg-/4 rnl.; BAL, i ^. ml. Similarly, isocitrate oxidation showed the same requirement for phosphate, and in the presence of oligomycin, for ADP, when respiration was stimulated by DNP. In this case it is not the accumulation of a-keto- glutarate, but the failure to produce sufiicient quantity of oxaloacetate, required for the coupling process, which is responsible for the low rates of oxygen consumption. The addition of malate, in catalytic quantities, abolishes the requirement for phosphate and ADP, for isocitrate oxidation. Succinate oxidation In confirmation of the findings of Azzone and Ernster [12] liver mito- chondria which had been pre-incubated with 2 mM arsenate and io~* m. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
Size: 1480px × 1688px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
