. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. REACTION OP THE HOST TO INFECTION 223 as it has been shown by Bordet that amboceptor does not unite with complement in the absence of antigen. It seems more probable that some other factor, such perhaps as a marked agglu- tmation of the bacteria in the stronger solutions, may serve to pro- tect them from the bacteriolytic action. Fixation of Complement.—As has been mentioned, it is pos- sible to produce cytolysins for red blo
. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. REACTION OP THE HOST TO INFECTION 223 as it has been shown by Bordet that amboceptor does not unite with complement in the absence of antigen. It seems more probable that some other factor, such perhaps as a marked agglu- tmation of the bacteria in the stronger solutions, may serve to pro- tect them from the bacteriolytic action. Fixation of Complement.—As has been mentioned, it is pos- sible to produce cytolysins for red blood cells. This is commonly done by injecting the washed blood corpuscles of a sheep ( + salt solution) into a rabbit intravenously three or four times at intervals of five days. The serum of the rabbit becomes. Pig. -Illustratmg the conception of deviation of complement. b, antigen; k, complement. a. Amboceptor; Strongly hemolytic for sheep's cells. The blood is drawn from the carotid artery, the serum separated, rendered perfectly clear and after heating to 56° C. for 30 minutes is stored in hermetically sealed ampoules containing i each, in a low temperature refrigerator. When this hemolytic amboceptor is diluted to the proper point, which must be ascertained by trial and error, it will just cause the complete hemolysis of a definite quantity of washed sheep's corpuscles ( of a 5 per cent suspension) when combined with of a 10 per cent solution of fresh normal serum of guinea-pig (complement). The mixture of this quantity of the immune serum, which may now be called one unit of hemolytic amboceptor, with of freshly prepared 5 per cent suspension of washed sheep's corpuscles produces a reagent which setves for the detection of complement and the approxi-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrat
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Keywords: ., bookcentury1900, bookdecade1920, booksubjectbacteri, bookyear1920
