. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMINO ACID UPTAKE IN TOAUK1SH LIVER 477 Free Protein aa-tRNA 12345 Time After Injection (minutes) FIGURE 3. Time course in the distribution of radioactive L-amino acids among various liver pools following hepatic portal vein injection in vivo at 24° in toadfish acclimated for two weeks at 11°, AiFr —intracellular free (cold trichloroacetic acid soluble) amino acid pool; Aipr —protein (hot acid insoluble) fraction; Ae—free amino acid pool attributed to extra- cellular space [from equation (4)]; Aiaa~tRN"A—aminoacyl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AMINO ACID UPTAKE IN TOAUK1SH LIVER 477 Free Protein aa-tRNA 12345 Time After Injection (minutes) FIGURE 3. Time course in the distribution of radioactive L-amino acids among various liver pools following hepatic portal vein injection in vivo at 24° in toadfish acclimated for two weeks at 11°, AiFr —intracellular free (cold trichloroacetic acid soluble) amino acid pool; Aipr —protein (hot acid insoluble) fraction; Ae—free amino acid pool attributed to extra- cellular space [from equation (4)]; Aiaa~tRN"A—aminoacyl transfer RNA. mated fish that had been fed continuously showed a reduced average AiMax of ± at 24°. Average polypeptide chain assembly time tc (or the average time for one round of protein synthesis on all active ribosomes) was determined for each group from the distribution of radioactivity between growing (ribosome-bound) and completed chains as a function of incubation time up to tc (Haschemeyer, 1969a). Values of tc obtained from incorporation of the L-amino acid mixture and of L-leucine alone are given in the last column of Table I. The results at 24° indicate a slightly higher synthetic rate than previously reported at 22°. Part of the difference is due to temperature; other contributing factors are a shorter period of starvation before experiment and a change in methodology that reduced loss during centrifugation of membrane-bound protein. Cold acclimated fish showed more rapid protein synthesis (smaller tc) at both experimental temperatures. Within each acclimation group Q10 for the process measured by tc (polypeptide chain elongation and release) was about 3. Net incorporation into protein , though subject to more individual variation, roughly paralleled the Protein. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
