. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 412 CHARIVA R \TANARAT-BROCKELMAN TABLE II Rate of Rhabditis maupasi population growth in three groups of cultures receiving separated protein fractions from Sephadex G-200. Control received only raw rabbit liver extract (four replicates}. > Control Cultures receiving fraction 3a 3b 3c Rate of population growth (r) Standard deviation 0* Per cent maturity 100 100 95 ** * Only two larvae were produced in one culture. ** remained undeveloped, and 17% developed to stage 4 larva
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 412 CHARIVA R \TANARAT-BROCKELMAN TABLE II Rate of Rhabditis maupasi population growth in three groups of cultures receiving separated protein fractions from Sephadex G-200. Control received only raw rabbit liver extract (four replicates}. > Control Cultures receiving fraction 3a 3b 3c Rate of population growth (r) Standard deviation 0* Per cent maturity 100 100 95 ** * Only two larvae were produced in one culture. ** remained undeveloped, and 17% developed to stage 4 larvae. There were significant differences in population growth rates among fractions ( = , P < ). offspring production during the 14 days of cultivation, whereas two generations were horn to the nematodes in control groups. The rates of population increase in nematode cultures receiving the two other protein fractions were significantly higher than the group receiving protein peak (Fo, 12 == , P < ). Thus, protein peak 3c evidently contained most of the inhibitor. Purification of inhibitor \ protein After the initial step of purification by rechromatographing the active peak 3c alone on a column of Sephadex G-200, the sample obtained was examined o CO (NJ UJ Gel filtration on Sephadex G - 200 Peak of activity. 3c 100 Elution volume 150 200 ml FIGURE 4. Gel filtration of active peak on a column of Sephadex G-200. Column dimension is > 120 cm; sample size, 2 ml ( mg protein); potassium phosphate buffer, M, pH ; ilo\v rate, 12 ml/hr; fraction volume, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Li
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
