. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Pyralidae 353 corrugations, which open to the sides, where they remain to pupate. Not more than 200 larvae should be put in each cage. Before being placed in the incubator the globes and their contents are immersed in water for from five to ten seconds. All excess water is drained off and the water absorbed by the cardboard supplies sufficient moisture for pupation. During the time of incubation it is necessary to look the material over two or three times a we
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Pyralidae 353 corrugations, which open to the sides, where they remain to pupate. Not more than 200 larvae should be put in each cage. Before being placed in the incubator the globes and their contents are immersed in water for from five to ten seconds. All excess water is drained off and the water absorbed by the cardboard supplies sufficient moisture for pupation. During the time of incubation it is necessary to look the material over two or three times a week and remove the dead larvae which would. LI- B D Fig. 67.—Oviposition cage for Pyrausta nubilalis. A, end view: b, frame; c. wood disc closing end of cage. B, front view with lower roller removed: d, upper roller; e, second roller; f, cylinder forming the cage; g, wire rods (4); w, wire screening; i, wire screening cylinder for feeding cotton. C, inside end view of cage. D, lower roller (hinged): r, wire rod. otherwise contaminate the healthy ones. The mortality among larvae varies between 5% and 10%. When overwintered larvae are used the moths begin to emerge in three weeks' time, but if using fresh larvae collected during the late summer and fall a much longer period of in- cubation is required. Emergence extends over a period of about 2 weeks. A cage for oviposition (Fig. 67) consists of a cylinder 12 inches long* and 5 inches in diameter suspended in a small frame (A, b) in a hori- zontal position by means of a central axle on which it revolves. The frame also supports a series of three rollers which hold the waxed paper in place. The cage is so constructed that its sides, which represent the greatest surface, are of waxed paper. The ends of the cage (A, c) are ?This dimension must correspond to the length of the roll of waxed paper used: not all rolls are 12 inches Please note that these images are extracted from scanned page images that may have been digitally
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