. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. (A) A neuron will: the characteristic morphology of n bipolar cell and showing positive labeling for serotonin iinmuno- reaclivity. This cell responded to 10/1 ^M GABA with an outward current when the voltage was clamped at -45 »iJ', no responses were elicited by either 100 nM kainate or 100 jiM glycine. Bar = 20 urn. (B) Spatial loctilnaiion of the response to GABA from a presumptive bipolar cell. The cell was most sensitive at the dendritic and cell body regions. The responses to GABA were greatly reduced by supe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. (A) A neuron will: the characteristic morphology of n bipolar cell and showing positive labeling for serotonin iinmuno- reaclivity. This cell responded to 10/1 ^M GABA with an outward current when the voltage was clamped at -45 »iJ', no responses were elicited by either 100 nM kainate or 100 jiM glycine. Bar = 20 urn. (B) Spatial loctilnaiion of the response to GABA from a presumptive bipolar cell. The cell was most sensitive at the dendritic and cell body regions. The responses to GABA were greatly reduced by supervision with 200 ^M /ricroto.\in (arrows), and showed partial recovery alter return to normal Ringer (not shown). (C) /-I 'profile ot the GABA response obtained Iroin another cell A voltage ramp was applied, and currents obtained in elasmobranch Ringer were subtracted from the currents recorded after the addition of GABA. In this case, the resultant GABA-induced responses reversed from an inward to an outward current at about - 76 in]'.. fixation, exposed to an antibody to serotonin, and reacted with an FITC-labelled secondary. Although positive labeling of the cells was often difficult to distinguish from background fluores- cence, six of the cells appeared to show labeling above back- ground: each of these cells had responded to superfusion of 100 AiMGABA. Supported by grants (EV-00777, EV-06516. and EV4W41 1) from the National Eye Institute. H. Ripps is a Research to Pre- vent Blindness Senior Scientific Investigator. Literature Cited 1. Malchow, R. P., et al. 1990. / Gen. Physwl 95: 177-198. 2. Rae, J., et al. 1991. J. NeurosciMethods 37: 15-26. 3. Malchow, R. P., et al. 1991. Biol. Bull 181: 323-324. 4. Lasater, E. M., el al. 1984. ./ \eumsei 4: 1966-1975. 5. Schlemermeyer, E., and R. L. Chappell. 1991. Invest. Ophthal. Vis. Sci. 32: Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - colorati
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