. Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically. ugation twoor three times in normal saline (), to render them neutral and abso-lutely free from serum. The whiteblood-corpuscles, being lighter thanthe red, will overlie the latter as agrayish layer of leucocytic cream (Fig. 24), whereupon they may bepicked up by a pipette and trans-ferred to another receptacle ( twenty-four-hour culture of thegiven bacterium is washed off in normal salt solu-tion (Fig. 27), then centrifuged to throw downall bacterial clumps
. Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically. ugation twoor three times in normal saline (), to render them neutral and abso-lutely free from serum. The whiteblood-corpuscles, being lighter thanthe red, will overlie the latter as agrayish layer of leucocytic cream (Fig. 24), whereupon they may bepicked up by a pipette and trans-ferred to another receptacle ( twenty-four-hour culture of thegiven bacterium is washed off in normal salt solu-tion (Fig. 27), then centrifuged to throw downall bacterial clumps. The supernatant suspensionshould contain only individual bacteria in not too V Fig. 22.—Capillaryglass capsules for thecollection of specimensof blood. A, simplestraight; B, Wrights. 26). A six- 236 APPLIED IMMUNOLOGY great concentration. The best strength would ap-pear to be that rendering a phagocytosis averag-ing about four bacteria pro leucocyte. Two opsoniz-ing capillary pipettes, P (patient) and C (control),are prepared so that the lumina are about the diame-ter of a large sized hat-pin. A distance of one-half. Fig. 27.—Washing the culture of the given bacterium from the culture medium, usingthe platinum loop, in order to obtain the bacterial suspension. or three-quarters of an inch is marked off on the endsof these two pipettes (Fig. 28,1 and II). By the useof a small rubber bulb, equal quantities of patientsserum, bacterial suspension and washed leucocytes,permitting a small bubble of air to separate each, aredrawn up and mixed by skilful thumb and fingergymnastics, in the pipette designated P. In the same * B a 3 an £ ° S sT o g cr o t-i- b, l-HO ffl T_ ?a ra a so .2 3 << _B £C- O C 3 £? S _ Ss! flS a < rt 2 hCbB lias H.£~er* ra E o -d
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherphila, bookyear1916
