. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. xlO^ 2 7 0 CELL NUMBER PER LITER -J 1 ' â¢â : 1 1 I I 1 i_ AVERAGE CELL VOLUME. 0 20 40 60 80 WO 120 HOURS IN THE LIGHT Fig. 4. Change of packed cell volume, cell nuniber, average cell volume and dark cell ratio durins; the transformation of dark cells into light cells. ^Measurements were made with the same material as that illustrated in Fig. 3. Fig. 3. Distribution polygons of cell diameter showing the transformation of active dark cells into l
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. xlO^ 2 7 0 CELL NUMBER PER LITER -J 1 ' â¢â : 1 1 I I 1 i_ AVERAGE CELL VOLUME. 0 20 40 60 80 WO 120 HOURS IN THE LIGHT Fig. 4. Change of packed cell volume, cell nuniber, average cell volume and dark cell ratio durins; the transformation of dark cells into light cells. ^Measurements were made with the same material as that illustrated in Fig. 3. Fig. 3. Distribution polygons of cell diameter showing the transformation of active dark cells into light cells in the light. Shaded portions of polygons indicate dark cells. The starting material of dark-cell-rich culture was prepared by growing the alga at 25", first under 10,000 lux light for I weak, then under 400 lux for 3 days. The culture was placed in the dark for i day, before the cells were transferred mto fresh medium for the main exj)criment. Main experiment was conducted at 9°-io~ C, with 2000 lux light, undi'r provision of COj-enriched air. I'rom other experiments, which we shall not reproduce here to save space, it was found that the transformation of dark cells into light cells in the light, at least within first 20 hours of illumination, was not affected markedly by the deprival of nitrogen or phosphorus or of whole nutrient salts from the culture medium. But later the transformation was more or less markedly affected by nutrient deficiency; in distilled water and in P-deficicnt medium it was halted completely at a certain stage, and in N-deficient medium it was increasingly retarded with the progress of incubation. It was also revealed that, during the course of transformation, the chlorophyll content of cells decreased, whereas the total amount of chlorophyll in the culture* increased in normal medium, but not in P- or N-free medium and in distilled water. * Calculated from the chlorophyll content of cells and the packed volume of cells contained
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Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
