. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. OSMOCONFORMITY IN ft THERMYDRON 169 Hydn mineral regulation Acclimation lime. To estimate the time necessary for hemolymph osmolality stabilization following a decrease in salinity, the crabs were first transferred from seawater (1002 mosm/kg), into a 740-mosm/kg medium. Hemolymph sam- ples were taken from surviving animals after 0, , 5, and 15 h in the dilute medium. As 75% of the animals were dead at 15 h and 100% shortly afterward, a second experiment was conducted in an 800-mosm/kg medium. Survival was 60% at 12 h an
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. OSMOCONFORMITY IN ft THERMYDRON 169 Hydn mineral regulation Acclimation lime. To estimate the time necessary for hemolymph osmolality stabilization following a decrease in salinity, the crabs were first transferred from seawater (1002 mosm/kg), into a 740-mosm/kg medium. Hemolymph sam- ples were taken from surviving animals after 0, , 5, and 15 h in the dilute medium. As 75% of the animals were dead at 15 h and 100% shortly afterward, a second experiment was conducted in an 800-mosm/kg medium. Survival was 60% at 12 h and 17% at 24 h. Hemolymph samples were taken from the surviving crabs after 0, , 3, 6, 12. 24. and 48 h. Osmotic regulation. The hemolymph osmolality of some crabs was measured as soon as they were brought on board. The crabs were then transferred to the different media, and their hemolymph osmolality was remeasured after a period of osmotic stabilization in each medium; the length of this period was determined from the results on adaptation time. A similar experiment was conducted under high pressure, at 15°C. The crabs were immersed in an 800-mosm/kg me- dium, in individual 400-ml containers set in a 19-1 pressur- ized tank called "Incubateur Pressurise pour 1'Observation en Culture d'Animaux Marins Profonds" (IPOCAMP) (Shillito, unpub.). The crabs were subjected for 13 h to a pressure of 260 bars, which approximates the pressure at the site of capture. The hemolymph was then sampled and its osmolality was measured. For sampling, the crabs were rinsed with deionized water and dried with absorbent paper. Hemolymph was sampled with a hypodermic needle mounted on a syringe and in- serted at the basis of a posterior pereiopod. The osmolality of a sample of hemolymph was immediately mea- sured on the Wescor Varro 5520 micro-osmometer. Ionic regulation. Hemolymph from the same samples was quickly diluted to 25% in deionized water, stored in Eppendorf tubes, and
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