. Bacteriophages. Bacteriophages; Bacteriophages. 498 BACTERIOPHAGES Tl plaques may not be distinguishable from the background ex- cept for the overlaps, so the plates should be examined after 5 or 6 hr. of incubation. A plate of mixed viruses on mixed indicator strains is illustrated in Fig. Figure 14. Mixture of phages Tl and T7 plated on mixed indicators B/1,5 and B/3,4,7. Note the 2 kinds of turbid plaques and clear overlapping areas of complete lysis. Bright field illumination. Applicaliun. A most important use of mixed indicator strains is in analysis of the phage yields from mixedl
. Bacteriophages. Bacteriophages; Bacteriophages. 498 BACTERIOPHAGES Tl plaques may not be distinguishable from the background ex- cept for the overlaps, so the plates should be examined after 5 or 6 hr. of incubation. A plate of mixed viruses on mixed indicator strains is illustrated in Fig. Figure 14. Mixture of phages Tl and T7 plated on mixed indicators B/1,5 and B/3,4,7. Note the 2 kinds of turbid plaques and clear overlapping areas of complete lysis. Bright field illumination. Applicaliun. A most important use of mixed indicator strains is in analysis of the phage yields from mixedly infected bacteria. If a bacterium is mixedly infected with 2 different viruses and then plated on the appropriate mixed indicator strains before the end of the latent period, 2 possible plaque types might be found. If the infected bacterium liberates both kinds of phages, a clear plaque will be formed. This is illustrated in the following Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Adams, Mark Hancock, 1912-1956. New York, Interscience Publishers
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