. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MATERIAL IN NERVES OF A JELLYFISH 81. Figure 2. Double labeling. (A) Whole mount of perirhopalial tissue ectoderm, stained with antibodies against taurine (red) and FMRFamide (green). The clear anatomical separation between the Tau-IR MNN neu- rons and FMRFamide DNN neurons is evident. (B) Endodermal tissues stained in the same manner. The majority of cells in this preparation are Tau-IR. Two neurons (arrows) were more (yellow) or less (green) intensely immunoreactive to FMRFamide, but at least one (arrowhead)
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MATERIAL IN NERVES OF A JELLYFISH 81. Figure 2. Double labeling. (A) Whole mount of perirhopalial tissue ectoderm, stained with antibodies against taurine (red) and FMRFamide (green). The clear anatomical separation between the Tau-IR MNN neu- rons and FMRFamide DNN neurons is evident. (B) Endodermal tissues stained in the same manner. The majority of cells in this preparation are Tau-IR. Two neurons (arrows) were more (yellow) or less (green) intensely immunoreactive to FMRFamide, but at least one (arrowhead) was reactive to both antibodies. Scale bars = 50 ^m. transmitter at these synapses. The current investigation provides immunocytochemical evidence that a taurine- like molecule is indeed present in MNN neurons and in an endodermal nerve net, but is not present, at least in detectable quantities, in neurons of another ectodermal nerve net, the diffuse nerve net (DNN). This was partic- ularly obvious in the double-labeling experiments, in which the ectodermal FMRFamide-IR was clearly re- stricted to the DNN (Anderson et ai. 1992). Although Tau-IR was also present in the ectodermal myoepithelial cells, its absence in the DNN indicates that taurine is not a constituent component of all nerve nets in this animal. This distinction is important because taurine acts as an osmoregulator in many marine organisms (Thurston et ii/., 1980). If it were serving the same function in Cyanea, one might expect it to be widespread in different cell types and present in all nerve nets. The presence of Tau-IR in the endodermal DNN in Cyiineu was unexpected considering that immunoreac- tivity to antibodies raised against the sea anemone neuro- peptide AnthoRFamide was found in these neurons (Anderson ct 1992). In the present study, endodermal Tau-IR neurons also were found to be immunoreactive to antibodies to FMRFamide. In addition, however, mor- phologically similar neurons in larger animals w
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