. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 140 K. V. SINGARAJAH AND F. I. HAROSI m u c o .£> _O H. —I— tee Wavelength (nm) -Q b 0 —i 1 1— 450 500 1 1 1— 550 600 Wavelength (nm) y 350 400 450 500 550 600 650 Wavelength (nm) Figure 2. Absorbance, bleaching difference absorbance, and linear dichroism spectra of the visual pigment in single cones of the black sea bass. A. Average absorbance spectrum based on four single-cell recordings ( + ). The solid curve is the result of Fourier smoothing. Peak absorbance and half-bandwidth are 464 nm and 4430 c
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 140 K. V. SINGARAJAH AND F. I. HAROSI m u c o .£> _O H. —I— tee Wavelength (nm) -Q b 0 —i 1 1— 450 500 1 1 1— 550 600 Wavelength (nm) y 350 400 450 500 550 600 650 Wavelength (nm) Figure 2. Absorbance, bleaching difference absorbance, and linear dichroism spectra of the visual pigment in single cones of the black sea bass. A. Average absorbance spectrum based on four single-cell recordings ( + ). The solid curve is the result of Fourier smoothing. Peak absorbance and half-bandwidth are 464 nm and 4430 cm"', respectively. B. Bleaching difference spectrum from one cell. Data values (O) were derived from prebleach and postbleach measurements consisting of the sum of 16 scans, each. The dashed curve is based on Fourier-smoothed data. The positive band peaks at 471 nm, with HBW = 3140 cm"1; the negative band peaks at 387 nm, with HBW = 3570 cm '. C. Average linear dichroism from 3 cells (A). The dotted line, again, is the result of Fourier- surements have been published previously (Harosi, 1987). The results for the black sea bass cones are listed in Table I. The last column in Table I shows numerical values for the transverse specific density, Si. For this determination, the transversely polarized component of the peak absor- bance, AI, is needed. The latter is derived from the average (unpolarized) peak absorbance. A, divided by factor f, which in turn depends on the dichroic ratio, R, defined as R = AI/AI,. Thus, f = (1 + R)/2R and Ax = A/f. Finally, S± = Ai/d, where d is the mean diameter of the compartment (Retry and Harosi, 1990). Thus, the mean- ing of S± is peak absorbance for transversely polarized light per unit thickness (measured either in micrometers or centimeters). Discussion In our teased preparations obtained from various re- gions of the retina of the black sea bass, we found rods, double cones, and single cones. The outer segment in each o
Size: 2219px × 1126px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
