. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MOLTING AND REGENERATION IN CRABS 223 lengthened for 10 to 14 days. Under these conditions, stimulation of growth (in the secondary regenerates) and inhibition of growth (in the remaining primary regenerates) are observed simultaneously in the same animal. The inhibition caused by the loss of regenerates is seen in animals stimulated to undergo precocious molts by either limb or eyestalk removal. Several preliminary reports of these results have been published (Holland and Skinner, 1974a, b). MATERIALS AND METHODS Animals Sp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MOLTING AND REGENERATION IN CRABS 223 lengthened for 10 to 14 days. Under these conditions, stimulation of growth (in the secondary regenerates) and inhibition of growth (in the remaining primary regenerates) are observed simultaneously in the same animal. The inhibition caused by the loss of regenerates is seen in animals stimulated to undergo precocious molts by either limb or eyestalk removal. Several preliminary reports of these results have been published (Holland and Skinner, 1974a, b). MATERIALS AND METHODS Animals Specimens of Gecarcinns latenilis were obtained from the Bermuda Biological Station or from J. Van Montfrans or W. Miley, Boca Raton, Florida. Precocious molts were initiated by either removal of both eyestalks or induction of autotomy of eight walking legs (Skinner and Graham, 1970, 1972). Autotomy is the phy- siological self-amputation of injured limbs at a preformed breakage plane by the abrupt contraction of a set of autotomy muscles in the basi-ischial segment (Wood and Wood, 1932). Animals autotomize limbs when threatened or when the merus segment of the limb is injured. Regeneration of the autotomized limbs occurs early in the premolt period [stage D0 (Skinner, 1962) and DI, these data]. The data on the size of the regenerating limb buds are expressed as a regeneration index (R), which is the length of the regenerate (measured at X magnification) divided by the maximal width of the carapace X 100 (Bliss, 1956). Because the length of regenerates of (R ^ 10) is difficult to determine precisely, these smallest R values are approximate. DNA synthesis For in -vitro studies, crabs were caused to autotomize regenerating limbs. The regenerates were rinsed several times in sterile M NaCl and placed in chambers containing Eagle's Minimal Essential Medium with electrolytes adjusted to concen- trations isotonic with Gccarcinns Jicnwlyinph (Skinner, Marsh and Cook, 1965) and 1
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
