Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . IX, 1065; Forster and Tomasczewski, Deutsch. , 1913, XXXIX, 1237; Wassermann, Ibid., 1281; Levaditi, Marie and Bankowski,Ann. de Ilnst. Pasteur, 1913, XXVII, 577; Nichols and Hough, Jour. Am. Med. Assn., 1913,LXI, 120; WUe, Ibid., 866. * Jour. Am. Med. Assn., 1913, LXI, 1504. * See McDonagh, Brit. Jour. Dermatol., 1912, XXTV, 381; and Ross, Lancet, 1912, II,1105. 58o DIAGNOSTIC METHODS. acteristic difiference between this spirochasta and some other types (spirochaetabuccal


Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . IX, 1065; Forster and Tomasczewski, Deutsch. , 1913, XXXIX, 1237; Wassermann, Ibid., 1281; Levaditi, Marie and Bankowski,Ann. de Ilnst. Pasteur, 1913, XXVII, 577; Nichols and Hough, Jour. Am. Med. Assn., 1913,LXI, 120; WUe, Ibid., 866. * Jour. Am. Med. Assn., 1913, LXI, 1504. * See McDonagh, Brit. Jour. Dermatol., 1912, XXTV, 381; and Ross, Lancet, 1912, II,1105. 58o DIAGNOSTIC METHODS. acteristic difiference between this spirochasta and some other types (spirochaetabuccalis), with which it might be confused, is that its ends lie above and belowa longitudinal line drawn through the center of its curvatures, while in the otherforms the ends lie on the projection of such a line. The organism moves in anoscillatory manner about its longitudinal axis, its movements being winding,bending and whipping, while in the spirilla the longitudinal axis remainsrigid. Schaudinn demonstrated the existence of a flagellum at each end, whilethe other spirochaete have an undulating Fig. 148.—Spirochsete pallidas and refringens. (Pitfield.)The darker ones are the refringens. These organisms are seen only with great difficulty in the specimens offresh blood, but thanks to the introduction of the ultra-condenser (the dark-field illuminator) we are in a position to see these organisms, both in the splenicand peripheral blood, although considerable practice is necessary properly toadjust the light. ^ These organisms do not take anilin dyes readily, so thatspecial methods have been advanced for their demonstration in smears. A verygood stain for them is the Goldhorn stain. The smears are fixed with puremethyl alcohol for 15 minutes and are then covered with the stain (polychromemethylene blue) for three to five seconds, when the excess is drained oflf. Thespecimens are then slowly introduced into clean water with the film sides the slide in this po


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