. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Isolation and Cultivation 411 the inner surfaces become flattened and separated from one another by a narrow interval. A pair of the cocci resembles a coffee-bean or a German biscuit, and is described by the Germans as semmelformig. The gonococci are small, the length of one of the coffee-bean cocci being about /u, its breadth about n. They are not motile, nor provided with flagella, and are without spores. Quite as characteristic as the form of
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Isolation and Cultivation 411 the inner surfaces become flattened and separated from one another by a narrow interval. A pair of the cocci resembles a coffee-bean or a German biscuit, and is described by the Germans as semmelformig. The gonococci are small, the length of one of the coffee-bean cocci being about /u, its breadth about n. They are not motile, nor provided with flagella, and are without spores. Quite as characteristic as the form of the organism is its rela- tion to the cells. In most of the inflammatory exudates the gono- cocci are contained either in epithelial cells or in leukocytes, very few of them lying free. This intracellular position is supposed to depend upon active phagocytosis of the cocci by the cells. It may not obtain in old lesions. Staining.—They stain readily with all the aqueous solutions of the anilin dyes—best with rather weak solutions, but not by Gram's Fig. 140.—Gonococci in urethral pus. The organisms contained in pus can be beautifully shown by first treating the prepared film with alcoholic eosin, and then with Lofifler's alkaline methylene blue. A differential color test can be made by staining the film by Gram's method and then with aqueous Bismarck brown, or, what may be still better, with 3 per cent, aqueous solution of pyronin. Ordinary pus cocci, taking the Gram's stain, appear blue-black; the gonococci, taking the counter- stain, are brown in the former, purplish red in the latter case. Isolation and Cultivation.—^The organism does not grow upon any of the ordinary culture-media, and grows very scantily upon any artificial medium. Wertheim* succeeded in by diluting a drop of gonorrheal pus with human blood-serum, mixing this with an equal part of melted 2 per cent, agar-agar at 4o°C., and pouring the mixture into Petri dishes, which, as soo
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
