. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 242 L. G. YOUNG AND L. NELSON 100 80 560 o :E 040 +-> c O 020. -20 -30 -50 Log [EDTA] FIGURE 4. Effect of EDTA on the swimming speed of Arbacia punctulata spermatozoa. EDTA added to filtered Woods Hole seawater containing 107 + 10% Arbacia punctulata spermatozoa per milliliter depresses the swimming speed at effective concentrations. The abscissa is in log molar concentration of EDTA in seawater/sperm suspensions. The ordinate is the swim rate of treated sperm expressed as per cent of the control rate
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 242 L. G. YOUNG AND L. NELSON 100 80 560 o :E 040 +-> c O 020. -20 -30 -50 Log [EDTA] FIGURE 4. Effect of EDTA on the swimming speed of Arbacia punctulata spermatozoa. EDTA added to filtered Woods Hole seawater containing 107 + 10% Arbacia punctulata spermatozoa per milliliter depresses the swimming speed at effective concentrations. The abscissa is in log molar concentration of EDTA in seawater/sperm suspensions. The ordinate is the swim rate of treated sperm expressed as per cent of the control rate in seawater. The points and ranges represent the mean and standard deviations of separate determinations made on spermatozoa collected from three individual Arbacia punctulata. Incubation temperature was 22-23° C. effect is negligible at 5 X 10~s M. Moreover, the stimulatory effect of HgClo on Arbacia sperm motility is transitory. The dashed lines in Figure 3 show the de- cline in swim speed observed after 16 and 24 minutes incubation in various concentrations of HgClo. For example, in S X 10~6 M HgClo motility is 170% above control level after the first centrifugation (8-minute measurement), 30% above control level after the second centrifugation (16-minute measurement) and 33% below control level after the third centrifugation (24 minutes measurement). Since an initial stimulation and subsequent decline in sperm motility was ob- served at all concentrations of HgClo tested, the effect of preincubation with mercuric ion on sperm swimming speed was determined. The results of a ten- minute preincubation of the sperm cells in 10~; to 10~7 M HgClo essentially con- firm the observations recorded on sperm cell delayed response illustrated in Figure Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biolo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
