Archive image from page 6 of A cytological study of haploid. A cytological study of haploid Crepis capillaris plants cytologicalstudy64holl Year: 1930 1930] Hollingshead: Study of Haploid Crepis Capillaris Plants 111 plasmic shrinkage. PMC's from the paraffin material were smaller than those in aceto-carmine as shown by the figures. Haidenhain's haematoxylin was used for both root tips and PMC's and iodine- gentian-violet, too, proved to be a useful PMC stain. When the latter was used it was found better to lengthen the time in the stain to an hour or so. Otherwise J. Clausen's schedule (Babc
Archive image from page 6 of A cytological study of haploid. A cytological study of haploid Crepis capillaris plants cytologicalstudy64holl Year: 1930 1930] Hollingshead: Study of Haploid Crepis Capillaris Plants 111 plasmic shrinkage. PMC's from the paraffin material were smaller than those in aceto-carmine as shown by the figures. Haidenhain's haematoxylin was used for both root tips and PMC's and iodine- gentian-violet, too, proved to be a useful PMC stain. When the latter was used it was found better to lengthen the time in the stain to an hour or so. Otherwise J. Clausen's schedule (Babcock and Clausen, 1929) was followed. The drawings were made with the help of a camera lucida using a Zeiss 90X apochromatic objective and a 20X compensating eye- piece giving a magnification of approximately 3000 and were reduced one-third in reproduction. Except where otherwise stated they are from aceto-carmine material. CYTOLOGICAL OBSERVATIONS Diploid and haploid chromosome complexes from root tips are shown in figure 3. One member of each pair of chromosomes can be readily distinguished in the haploid complex. They behave normally in division. Similar complexes were observed in the walls of the ovaries of the haploid plants. The possibility that the chromosomes in haploids are smaller than those in diploids was mentioned in the preliminary note (1928). Further observations on this point were unconvincing. X Fig. 3. Somatic metaphases from root tips of diploid and haploid Crepis capillaris plants. As stated above, diploid cells and even wholly diploid root tips were found in the haploid plants. The number of root tips examined from each plant, the number in which some diploid metaphases were found, and the number in which only diploid metaphases were seen, is given in table 1. Of the 110 root tips of all plants examined, 28 showed at least one diploid metaphase in otherwise haploid tissue, and in 42 root tips only diploid metaphases were seen. Since the number of dip
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