. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 326 R. D. GATES ET AL. Figure 2. Left panel: photomicrographs of the host cells released to the seawater by Aiptasia pulchella in response to cold stress, stained for viability with fluorescein diacetate (X4000). Right panel: photomicro- graphs of the host cells released to the seawater by Pocillopnra damicornis in response to cold stress, stained with the DNA specific fluorochrome Hoechst 33258 (X4000). branches were immediately transferred to beakers con- taining 25 ml of MFSW at ambient temperature (23- 24°C). The beakers
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 326 R. D. GATES ET AL. Figure 2. Left panel: photomicrographs of the host cells released to the seawater by Aiptasia pulchella in response to cold stress, stained for viability with fluorescein diacetate (X4000). Right panel: photomicro- graphs of the host cells released to the seawater by Pocillopnra damicornis in response to cold stress, stained with the DNA specific fluorochrome Hoechst 33258 (X4000). branches were immediately transferred to beakers con- taining 25 ml of MFSW at ambient temperature (23- 24°C). The beakers were placed in the seawater tables for ambient temperature control and the coral tissue and sea- water in the beakers was sampled after 12 h. Controls for both species were treated identically to experimental an- imals but were maintained at ambient seawater temper- ature (25°C for A. pulchella and 23-24°C for P. dami- cornis) for h. Heat stress. Individuals of A. pulchella were placed in Petri dishes (35 X 10 mm) containing 4 ml of MFSW warmed to 32°C. Small branches of P. damicornis (2-3 cm length) were placed in beakers containing 25 ml MFSW pre-heated to 32°C. The animals were maintained at this temperature for up to 16 h. The water surrounding experimental specimens was examined microscopically at hourly intervals and the cellular entity released to the seawater removed and treated as described below. Control animals of both species were maintained at ambient sea- water temperature (25°C for A. pulchella and 23-24°C for P. damicornis) over the experimental time period. Staining and epijluorescence microscopy The cellular entities released during and after temper- ature stress were collected with a fine bore mouth suction pipette and deposited onto coverslips coated with poly- L-lysine ( in distilled water). The entities were stained for viability with the fluorogenic dye fluorescein diacetate (Sigma Chemical Co., stock solution 15 mg/ml in acetone; work
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