. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. RED ABALOXE REPRODUCTION 299 Raven (1966) reviewed the cytology, cytochemistry, and viteUoo-ene- sis of mollnscan oogenesis. More recently, with the electron microscope, Bolognari (1961a) illnstrated two different methods of yolk formation in gastropods. Tomita (1967) reported on the development of eggs in Haliotis discus hannai and classified egg development into a number of stages on the basis of tlu> most prominent feature of th
. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. RED ABALOXE REPRODUCTION 299 Raven (1966) reviewed the cytology, cytochemistry, and viteUoo-ene- sis of mollnscan oogenesis. More recently, with the electron microscope, Bolognari (1961a) illnstrated two different methods of yolk formation in gastropods. Tomita (1967) reported on the development of eggs in Haliotis discus hannai and classified egg development into a number of stages on the basis of tlu> most prominent feature of the cell in a ])ar- ticular stage. MATERIALS AND METHODS From Mareli 1968 through March 1969, ten subtidal red abalone specimens were collected monthly near Fort Bragg, California, either with scuba or by free diving. The depths from wliich abalone were taken ranged to 50 ft. The sliell of each specimen was removed and measured lengthwise in millimeters. The sex was recorded. The gonad was sampled in Uvo areas, near the tip and near the midportion. Tlie latter area was determined by finding the tangent of the gonadal-cone parallel to the longitudinal axis of the animal as the cone lay around the columellar muscle (Figur(> 1). The samples were fixed in a mixture of formalin, acetic acid, and ethyl alcohol. Portions of each sample were dehydrated in isopropyl alcohol, embedded in paraffin, sectioned at 7 microns, and stained with Eiirlich's hematoxvlin and FIGURE 1—Haliofis rufescens with shell removed, showing conical appendage (stippled) and sampled areas of the appendage (broken lines). Midportion determined by find- ing tangent of the cone parallel to the longitudinal axis as shown by heavy line. The gonad bulk index (Newman, 1967) and the gonad index (Boo- lootian, et al. 1962) were determined for each specimen from the sam-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and
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