. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CRAB HATCHING SUBSTANCE 177. 1mm 1mm Figure 1. A method for assessing the activity of OHSS. (a) One of the setal segments (ss) subdivided before hatching, with its developing embryos (cm) still attached, (b) An ovigerous seta is cut into six pieces (1-6). The arrow shows where the tip of the seta is removed (see text for details). This figure shows a seta just after the zoeas have hatched, leaving the empty egg cases (<r) still attached to ovigerous hairs which, however, are visible, (c) Ovigerous hairs (oh) incubated in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CRAB HATCHING SUBSTANCE 177. 1mm 1mm Figure 1. A method for assessing the activity of OHSS. (a) One of the setal segments (ss) subdivided before hatching, with its developing embryos (cm) still attached, (b) An ovigerous seta is cut into six pieces (1-6). The arrow shows where the tip of the seta is removed (see text for details). This figure shows a seta just after the zoeas have hatched, leaving the empty egg cases (<r) still attached to ovigerous hairs which, however, are visible, (c) Ovigerous hairs (oh) incubated in 10 ppt SW. and the attached embryos then pulled gently away with forceps, (d) The same, but the ovigerous hairs (oh) incubated in hatch water. Note that many of the hairs incubated in seawater are broken off (compare c and d). was compared with that of undiluted samples. This ex- periment was carried out with hatch water of S. haema- tochcir and S. picluin. Appearance of OHSS activity in living females To determine when OHSS is released, either by the larvae or by the female, one or two ovigerous setae were detached from the female before and after hatching. Before hatching, unhatched embryos were attached to the setae, but after the release of larvae, only the remnants ( broken egg capsules, funiculi. and the investment coat) remained on the setae. These setae were subdivided, and the embryos or the remnants were pulled with forceps. Time of hatching and larval release was determined and recorded by the photoelectric switch method described earlier (Saigusa, 1992, 1993). Presence of OHSS activity before hatching Embryonic development of 5. haematocheir can be di- vided into four stages by visual inspection: the early stage (10 days), from the start of cleavage to just before the formation of eye pigments; the intermediate stage (10 days), from eye pigment formation to completion of the compound eyes; the late stage (1 week), in which the egg color changes from brown to br
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
