. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 104 K. R^ CASTLEMAN Since the entire floor of the color cylinder is the single color black, some authors prefer to consider the HSI color space to be biconic. There are several variations of the HSI conversion for- mulas (Pratt, 1991), but the particular one chosen usually will not materially affect the result, as long as (1) intensity is a weighted average of R, G, and B: (2) hue is an angle; (3) hue, saturation, and intensity are independent; and (4) the transformation is accurately invertible. Multispectral images having
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 104 K. R^ CASTLEMAN Since the entire floor of the color cylinder is the single color black, some authors prefer to consider the HSI color space to be biconic. There are several variations of the HSI conversion for- mulas (Pratt, 1991), but the particular one chosen usually will not materially affect the result, as long as (1) intensity is a weighted average of R, G, and B: (2) hue is an angle; (3) hue, saturation, and intensity are independent; and (4) the transformation is accurately invertible. Multispectral images having more than three primary colors can also be transformed into corresponding multi- dimensional color spaces. For example, one can convert from rectangular to spherical coordinates in a color space based on four or more primary colors. Color Compensation Available stains and fluorophores have different, often broad absorption and emission spectra, and commonly used color cameras have broad, overlapping sensitivity spectra. As a result, one seldom obtains complete isolation of three types of objects in the three component Figure 5. Three-color, fluorescently labeled bone marrow cells. (A) Color image; (B) red; (C) green; (D) blue. Notice that all structures appear in each of the R, G. and B Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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