. A manual of bacteriology for agricultural and general science students. Bacteriology. CULTUEE METHODS 26 Exercise 37. Preparation of Dunham's Solution (for Indol Tests) Peptone . Sodium chloride Distilled water . g. g. cc. r~\ Dissolve, place in test tubes, and sterilize as usual. Ten days after inoculation, test for indol as follows: add 1 cc. of per cent solution potassium nitrite and a few drops of concentrated sulphuric acid. Warm gently by putting tubes in warm water. A pink color indicates the presence of indol. The action of an excess of sulphuric acid on peptone ca
. A manual of bacteriology for agricultural and general science students. Bacteriology. CULTUEE METHODS 26 Exercise 37. Preparation of Dunham's Solution (for Indol Tests) Peptone . Sodium chloride Distilled water . g. g. cc. r~\ Dissolve, place in test tubes, and sterilize as usual. Ten days after inoculation, test for indol as follows: add 1 cc. of per cent solution potassium nitrite and a few drops of concentrated sulphuric acid. Warm gently by putting tubes in warm water. A pink color indicates the presence of indol. The action of an excess of sulphuric acid on peptone causes a brown color in the solution. Exercise 38. Preparation of Phenol Bouillon Add 1 g. of phenol (carbolic acid) crystals to 1 liter of beef bouillon pre- pared as directed in Exercise 8. This medium is more frequently used for the cultivation or isolation of B. coli. A solid medium may be had by the addition of gelatin or Fig. 15. A fermentation tube Exercise 39. Preparation of Lactose Bile for Isolation of Intestinal Bacteria 1. Procure from the slaughterhouse an ox gall bladder. Empty the bile into a graduated cylinder and add 1 per cent of lactose. 2. Fill fermentation tubes and sterilize them in the Arnold sterilizer. Exercise 40. Preparation of Neutral Red Broth Prepare beef bouillon in the regular way, adding 1 per cent lactose, and to each 100 cc. of bouillon add 5 cc. of a 1 per cent solution of neutral red (Griibler's Neutral Roth nach P. Ehrhch). Tube and sterilize in the Arnold Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Reed, Howard Sprague, 1876-. Boston, New York, Ginn
Size: 1550px × 1611px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1914
