. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 130 T. c. LAJEUNESSE AND R. K. TRENCH californium produced a single restriction pattern character- istic of "clade B" Symbiodinium (Rowan and Powers, 1991) (data not shown). The utility of RFLP analyses of the SSUrDNA in distinguishing sequence variation, especially among congeneric members, has limitations. The more vari- able gene regions and more informative techniques were therefore employed to achieve a better resolution of the variation detected by the Taq 1 enzyme. An 850-bp fragment of the LSUrRNA gene was
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 130 T. c. LAJEUNESSE AND R. K. TRENCH californium produced a single restriction pattern character- istic of "clade B" Symbiodinium (Rowan and Powers, 1991) (data not shown). The utility of RFLP analyses of the SSUrDNA in distinguishing sequence variation, especially among congeneric members, has limitations. The more vari- able gene regions and more informative techniques were therefore employed to achieve a better resolution of the variation detected by the Taq 1 enzyme. An 850-bp fragment of the LSUrRNA gene was exam- ined by restriction analysis in an attempt to measure the extent of the genetic varitation observed in our RFLP anal- ysis of the small subunit and to uncover possible variation not resolved by the SSUrRNA gene (Baker el 1997; Wilcox, 1998). Dpn II restriction digests of LSUrDNA amplified from all northern Symbiodinium populations pro- duced the fragment pattern in Figure 1A, lane 2. A restric- tion site exists at one end of the amplified product and produces two bands, one 740 bp, the other about 70-80 bp. A Dpn II digest of the LSUrDNA from S. californium lacks a restriction site for this enzyme, and a single band is depicted (Fig. 1A. lane 3, and also 1C. lane 4). RFLPs conducted on algal populations from southern anemones always contained a nondigested fragment, as observed for S. californium, as well as two fragments identical to those observed in restriction patterns from the northern algal populations. This indicates the presence of two different gene sequences in the same amplification product—one with and one without a restriction site for Dpn II. These data are consistent with our results from the SSUrDNA digests. Taq 1 digests of the LSUrDNA (data not shown) also showed the presence of a single type in northern populations and two types in southern populations of A. elegantissima. The same two algal types were also found in A. .\antho- grammica from Cayu
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