. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I 2 FIGURE 4. Ouchterlony Petri dish double diffusion method for the analysis of antigens in yolk and "ghost" material. Well contents: A, NaCl-solubilized YP; B, CaCl»-solubilized YP; C, antiserum. Analysis 1: chicken antiserurn (anti-YPca) in well C. Note five bands of antigen-antibody precipitate with YPnn, four bands with YPca. Analysis 2: rabbit antiserum (anti-"ghost") in well C. Controls (control serum in well C) negative for both analyses 1 and 2. longed incubation with calcium ions. Differences in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I 2 FIGURE 4. Ouchterlony Petri dish double diffusion method for the analysis of antigens in yolk and "ghost" material. Well contents: A, NaCl-solubilized YP; B, CaCl»-solubilized YP; C, antiserum. Analysis 1: chicken antiserurn (anti-YPca) in well C. Note five bands of antigen-antibody precipitate with YPnn, four bands with YPca. Analysis 2: rabbit antiserum (anti-"ghost") in well C. Controls (control serum in well C) negative for both analyses 1 and 2. longed incubation with calcium ions. Differences in ionic strengths required for lysis, turbidity of the lysed yolk solutions, ease of denaturation, and kinetics of precipitation upon dilution all suggest a difference between the mechanisms of solubilization by NaCl and CaGi>. Nevertheless, short exposure of solubilized yolk to calcium does not permanently alter its solubility characteristics, since the dilution precipitate of freshly calcium-lysed yolk can be dissolved in M NaCl and will yield either YP or YC at the proper salt concentrations. The different types of precipitate that form upon dilution of NaCl-lysed yolk (YP, YC, and WC) were at first thought to represent different protein fractions. However, since each of these "fractions" can be redissolved in M NaCl and YP, YC and WC material produced from any one of these fractions, it is likely that they merely represent different physical states of the same yolk components. This apparent similarity is further verified by the electrophoretic analyses reported here. Both YC and yolk platelets are dense materials, and YC was found to be identical in volume to the platelets from which it was derived. This similarity in volume indicates that the components of YC may be organized as in yolk platelets. The ability to transform viscous YC to a rigid, platelet-like material by a reduction in salt concentration also indicates a similar macromolecular packing i
Size: 1581px × 1581px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
