. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 372 JAMES S. McDANIEL RESULTS Temperature and pH changes, alone or sequentially applied, had no direct effect on excystment. Excystment did not occur in Ringer at pH 1 (Ringer-HCl) or pH 7 at room temperature or 37° C. during a 10-hour period, nor during 5 hours at pH 7 after as much as 5 hours pre-exposure to pH 1. Saturated pancreatin, 1% pancreatin, \% trypsin, sodium taurocholate and M cysteine had no 100 o» CO o* 60 20. 15 45 Minutes 75 FIGURE 3. Excystment after gastric treatment. Conditions: \% pepsin (pH 2)
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 372 JAMES S. McDANIEL RESULTS Temperature and pH changes, alone or sequentially applied, had no direct effect on excystment. Excystment did not occur in Ringer at pH 1 (Ringer-HCl) or pH 7 at room temperature or 37° C. during a 10-hour period, nor during 5 hours at pH 7 after as much as 5 hours pre-exposure to pH 1. Saturated pancreatin, 1% pancreatin, \% trypsin, sodium taurocholate and M cysteine had no 100 o» CO o* 60 20. 15 45 Minutes 75 FIGURE 3. Excystment after gastric treatment. Conditions: \% pepsin (pH 2) for 30 minutes at 37° C., removed to 1% trypsin plus sodium taurocholate at pH 7 (O—O), • — • plus 5% CO2 atmosphere, rj—Q plus M cysteine, A—A plus M cysteine and 5% COs atmosphere. effect when tested singly. The response with pepsin depended upon its concen- tration, pH and temperature (Table I). Some excystment was initiated by exposure to conditions of the small intestine without pretreatment with gastric conditions (Fig. 1). Natural fluids from Lams argcntatus (herring gull) were tested also. Several dilutions (1:4, 1:9, 1:14) of fresh bile were not effective, but incubation in intestinal juice led to 75% excystment within 45 minutes (Fig. 1). Incubation at room temperature failed to. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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