A manual of the modern theory and technique of surgical asepsis . Fig. 13.—Diluting cultures. either by means of the hanging drop, that is,by placing a drop of the sterilized nutritive liquid,with the aid of a sterilized platinum wire, upon a. Fig. 14.—Founding plates. sterilized cover-glass to which a small quantity ofthe culture is added; or the nutritive gelatin,after being mixed with the cultures and beingliquefied in a test-tube, is poured on sterilized INFLUENCE OF MICROBES. 33 glass plates (Petris plates; Fig. 14), where themicrobes will grow within a day or two. Of especial importance
A manual of the modern theory and technique of surgical asepsis . Fig. 13.—Diluting cultures. either by means of the hanging drop, that is,by placing a drop of the sterilized nutritive liquid,with the aid of a sterilized platinum wire, upon a. Fig. 14.—Founding plates. sterilized cover-glass to which a small quantity ofthe culture is added; or the nutritive gelatin,after being mixed with the cultures and beingliquefied in a test-tube, is poured on sterilized INFLUENCE OF MICROBES. 33 glass plates (Petris plates; Fig. 14), where themicrobes will grow within a day or two. Of especial importance are the needle-punc-tures or needle-point cultures (Fig. 12, a) andthe linear cultures (Fig. 12, b). The first areobtained by bringing a platinum wire into con-tact with a particular colony and then plungingit into gelatin which has been put into a test-tube,the cultures appearing in the area of the punc-ture obtained by drawing the wire lightly overthe surface of the hardened gelatin. If certain pathological changes make their ap-pearance in the test-animals, these changes mustbe proven to have been caused entirely by mul-tiplication of the inoculated microbes. Further-more, the cultivation of one specimen of microbetaken from the i
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectsurgery, bookyear1895
