. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 202 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Reference: Biol. Bull 199: 202-203. (October 2000) Effects of Myosin-II Antibody on Actin-Dependent Vesicle Transport in Extracts of Clam Oocytes Leslie Sandberg, Phillip Stafford and George M. Langford (Dartmouth College, Hanover, New Hampshire 03755) The movement of vesicles and organelles in cells has been shown to occur on both microtubules and actin filaments (1. 2). The transport of vesicles on microtubules permits movement over long distances in cells, while the sho
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 202 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS Reference: Biol. Bull 199: 202-203. (October 2000) Effects of Myosin-II Antibody on Actin-Dependent Vesicle Transport in Extracts of Clam Oocytes Leslie Sandberg, Phillip Stafford and George M. Langford (Dartmouth College, Hanover, New Hampshire 03755) The movement of vesicles and organelles in cells has been shown to occur on both microtubules and actin filaments (1. 2). The transport of vesicles on microtubules permits movement over long distances in cells, while the short-range transport of \esicles on actin filaments produces the fine and precise movements re- quired to position vesicles at membrane sites for capture, docking, or fusion (3. 4). The transport of vesicles on actin filaments requires vesicle-associated myosins. some of which have been identified. Myosin V, for example, transports endoplasmic reticu- lum (ER) vesicles (5-7) and synaptic vesicles (8) in neurons and is involved in the capture of melanosomes at the tips of mouse melanocytes (9). In addition, myosin V is involved in the transport of lysosomes in some cells (10), secretory vesicles in yeast (II). and melanosomes in Xenopus melanophores (12). Myosin II, also known as conventional myosin. is vesicle- associated in extracts of clam (Spisula solidissima) oocytes (4. 13). In addition, myosin II was reported to be associated with Golgi vesicles (14), but the antibody used in these studies cross-reacts with coatomer proteins on Golgi-derived vesicles (15-17). There- fore, the role of myosin II in vesicle transport remains controver- sial. In this study we performed antibody-inhibition experiments with extracts of clam oocytes to provide additional evidence that myosin II is a vesicle motor. Clam oocyte extracts are well characterized and have been used extensively to study cell cycle events including cyclin synthesis and degradation, as well as ubiquitin-mediuted proteolysi
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