The Journal of biological chemistry . lood sugar estimations were made by the Shaffer-Hartmannmethod (3). The primary effect of the administration of the earlier extractsto normal rabbits was a great rise in the concentration of bloodsugar. This effect soon passed off, however, and marked hypo-glycemia followed. Also the earlier extracts were decidedly toxicin nature and the animals were extremely weak for some hoursfollowing the injection. Some died apparently as a direct resultof toxic substances present in the injected solution. It was foundthat a good deal of the toxic action could be over
The Journal of biological chemistry . lood sugar estimations were made by the Shaffer-Hartmannmethod (3). The primary effect of the administration of the earlier extractsto normal rabbits was a great rise in the concentration of bloodsugar. This effect soon passed off, however, and marked hypo-glycemia followed. Also the earlier extracts were decidedly toxicin nature and the animals were extremely weak for some hoursfollowing the injection. Some died apparently as a direct resultof toxic substances present in the injected solution. It was foundthat a good deal of the toxic action could be overcome by usingrelatively weak extracts. Here, however, the hypoglycemiawhich developed was not so striking. J. B. Collip 515 The results of a number of experiments in which extracts ofvarious types prepared from various sources were administeredby subcutaneous injection to normal, well fed rabbits and thesubsequent effect of the injection upon the blood sugar are shownin the form of protocols and charts. 003 HP3 Chart IYeast extract I. --© Time Chart I. Results of Experiments with Yeast Extract I. The results of four experiments in which yeast extract I was usedare indicated in Chart I. It will be noticed that the low points forblood sugar were observed to occur from 10 to 24 hours followingthe injection of the yeast extract. This is a point of great differ-ence from the hypoglycemia following the administration ofinsulin. The writer has obtained^ however, delayed actionwith certain pancreatic extracts. These results have been re-ported elsewhere (4). Rabbit 1, Chart I, was killed 23| hoursafter the injection had been made in order to estimate the glyco-gen content of the liver. This substance was completely lackingin that organ. Here again, however, a rabbit which has received 516 Glucokinin insulin may on occasion be found to have little or no glycogen inthe liver. This experiment would have been much more con-vincing if the liver had contained glj^cogen. The blood sugarcurve
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