. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 522 A. K. PAR TART. W. R. AMBERSOX, AND D. R. STEWART XaCl-f- N~a,IIPO4 (in the ratio 14 parts XaCl to 1 part Xa,l I!'(.), i at pi I All dilutions have been made with a salt solution of the same concentration. Changes in hydrogen ion concentration have no effect upon the read- ing between pi 1 and (determinations in steps of pH units) nor does the presence of 1 per cent HC1 alter the reading. In a like manner oxygenation or reduction has no effect. This might be antici- pated from the transmission c
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 522 A. K. PAR TART. W. R. AMBERSOX, AND D. R. STEWART XaCl-f- N~a,IIPO4 (in the ratio 14 parts XaCl to 1 part Xa,l I!'(.), i at pi I All dilutions have been made with a salt solution of the same concentration. Changes in hydrogen ion concentration have no effect upon the read- ing between pi 1 and (determinations in steps of pH units) nor does the presence of 1 per cent HC1 alter the reading. In a like manner oxygenation or reduction has no effect. This might be antici- pated from the transmission characteristics of the green filter used. The plasma introduced with the whole blood in preparing the dilute solutions of hemoglobin constitutes a small but variable source of error. This error appears to be partly the result of a slight degree of hemolysis which takes place in the whole blood upon standing, though it is kept at about 2° C. until used. The error varies between 1 and 4 per cent, and as it is determinable, a correction can be applied. This factor, due to pigments in the plasma, ma}- be determined by suspension of whole 380. 12 15 18 21 24 27 30 4. Shift with time of the transmission of dilute hemoglobin solutions (dilution 1 to 2,000). Ordinates represent readings of milliammeter; abscissae, time elapsed since the preparation of the hemoglobin solutions. blood in isotonic salt solution in the same dilution as that used to pre- pare the hemoglobin solution. The cells are then removed by centri- fuging. The supernatant fluid is read by the pyrometer and compared with the value for distilled water. Another important factor influencing the determination in dilute solutions of hemoglobin is the time elapsed since the preparation of the solution. Readings were taken on samples of the same solution at a ics of time intervals after its preparation. In Fig. 4 the results of two such experiments are plotted. Between the time of preparation of tin- solution and 4 to 8
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
