. Analysis of development. Embryology; Embryology. Gametogenesis, Fertilization and Parthenogenesis 189 Carbohydrate was later found in purified fer- tilizin (Tyler, '42) and in electrophoretically homogeneous gelatinous coat (Runnstrom et al., '42) of sea urchins. From this and later work (Tyler, '48a,b, '49; Krauss, '49; Vasseur, '48a,b, '49) it has become clear that fertilizin is a complex of amino acids and sugars that may be termed a glycoprotein. A procedure for the preparation of elec- trophoretically homogeneous sea urchin fer- tilizin is outlined in Table 11. Some of the analytical da
. Analysis of development. Embryology; Embryology. Gametogenesis, Fertilization and Parthenogenesis 189 Carbohydrate was later found in purified fer- tilizin (Tyler, '42) and in electrophoretically homogeneous gelatinous coat (Runnstrom et al., '42) of sea urchins. From this and later work (Tyler, '48a,b, '49; Krauss, '49; Vasseur, '48a,b, '49) it has become clear that fertilizin is a complex of amino acids and sugars that may be termed a glycoprotein. A procedure for the preparation of elec- trophoretically homogeneous sea urchin fer- tilizin is outlined in Table 11. Some of the analytical data obtained (Tyler, '48a,b, '49) on such preparations of fertilizin of the sea Upon electrophoresis the fertilizins of E. cordatum (Runnstrom et al., '42) and of 5. purpuratus (Tyler, '48a, '49) migrate towards the anode at pH values as low as pH 2. This highly acidic character is prob- ably correlated with the high content (ca. 25 per cent) of sulfate found by Vasseur ('47) in E. esculentus and confirmed by Ty- ler ('48b) in S. purpuratus. Upon ultracen- trifugation of a jelly coat solution of Psammechinus, Runnstrom et al. ('42) found a main component with a sedimentation constant of X lO"!^ varying with con-. T< Fig. 59. Eggs of the sea urchin Strongylocentrotus purpuratus, photographed in Syracuse dishes, X Via- a. Un- treated egg-suspension. b,\S minutes after addition of a solution of antifertilizin. (From Tyler, '40b.) urchin Strongylocentrotus purpuratus are given in Table 12. The values given there for amino acids and reducing sugars are probably low, since some of these very likely contribute to the humin residue that forms upon acid hydrolysis. By means of paper chromatography Vasseur and Immers ('49) find differences in the sugar components of the hydrolyzed jelly coat of different species of sea urchins; namely galactose in Echinus esculentus, fucose in Echinocardium corda- tum, fucose and galactose in Strongylocen- trotus droebachiensis, fucose and glu
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