. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 Gonadal stage Figure 3. Average serotonin concentration in surf clam gonads during the gametogenic cycle. Data from both sexes are combined. The gameto- genic cycle was divided into four stages: (1) active phase. (2) ripe phase. (3) partially spawned phase, and (4) spent phase. The letters a and b indicate relationships among the stages. The two stages (1 and 4) marked "a" are not different from each other, but they are significantly different from the stage (2) marked "b". The stage (3) marked "
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 234 Gonadal stage Figure 3. Average serotonin concentration in surf clam gonads during the gametogenic cycle. Data from both sexes are combined. The gameto- genic cycle was divided into four stages: (1) active phase. (2) ripe phase. (3) partially spawned phase, and (4) spent phase. The letters a and b indicate relationships among the stages. The two stages (1 and 4) marked "a" are not different from each other, but they are significantly different from the stage (2) marked "b". The stage (3) marked "ab" is not signifi- cantly dillerent from any of the three other stages. Vertical error bars correspond to the standard error of the means (SEM). females, the staining around acini was more frequently interrupted (Fig. 6B), and it was concentrated in varicosities within 5-HT-IR fibers (Fig. 7B). It was also noted that staining was observed neither on or in oocytes present in gonad sections (Fig. 7B) nor in isolated oocytes (not shown). In the subsequent stage, when spawning had begun and acini contained fewer ripe oocytes in their lumen, different patterns of 5-HT-IR fibers could be seen (Figs. 6C, c 1500 o ro fc 1200 0) c 5 900 •| ~~ 600 o 8 300 o </) 0. 234 Gonadal stage 1234 Gonadal stage Figure 4. Average serotonin concentration in surf clam gonads during female and male gametogenic cycle. The gametogenic cycle was divided into four stages: (1) active phase. (2) ripe phase. (3) partially spawned phase, and (4) spent phase, for both females ( 9 ) and males < 6 ). The only significant differences were found in females between gonadal stages I and 2. I and 3. 2 and 4. and .1 and 4. The letters a and b indicate relationships among the stages. The two stages marked "a" are not different from each other, but they are significantly different from the other two stages marked "b". In males, none of the stages were significantly different one from ano
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