. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NrXRIENT MEDIA AND METHODS OF CULTIVATION. 105 After the final treatment in the steam steriKser some of the tubes of gelatine and agar-agar are placed upright and allowed to set, and others are placed on an inclined plane or in the blood-serum inspissator, and left to gelatinise with an oblique surface. (b) Methods of employing Nutrient Jelly in Test-tubes AND ON Glass Plates. Test-tube-cultivations.—To inoculate test-t
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. NrXRIENT MEDIA AND METHODS OF CULTIVATION. 105 After the final treatment in the steam steriKser some of the tubes of gelatine and agar-agar are placed upright and allowed to set, and others are placed on an inclined plane or in the blood-serum inspissator, and left to gelatinise with an oblique surface. (b) Methods of employing Nutrient Jelly in Test-tubes AND ON Glass Plates. Test-tube-cultivations.—To inoculate test-tubes containing nutiient jelly, the cotton-wool plug is first twisted round in case there are an}' adhesions between the plug and the test-tube. It is then removed with the thumb and index finger of the right hand, and placed between the fourth and fifth fingers of the left hand, instead of being put down on the laboratory table and thereby probably contaminated with bacteria oi' the spores of mould fungi. A steriHsed needle charged, for example, with blood or pus con- taining bacteria, oi' with a colony from a. plate-culture, is thrust once in the middle line into the nutrient jelly, and steadily withdrawn. The tube should be held horizontally or witli its mouth downward, to avoid, as far as possible, accidental contamination from the gravitation of gei'ms in the air; and the plug replaced as quickly as possible. The cotton-wool project- ing bej'ond the mouth of the tube is then thoroughly bui'nt in the flame of a Bunsen burner oi' blow- pipe, and an india-rubber cap fitted over the mouth of the tube The chances of error arising from contamination of the culti- vations are reduced by avoiding di'aughts at the time of inoculation, and it is best that these manipulations should be carried on in a quiet room in which the tables and floor are wiped with damp cloths, rather than in a laboratory in which the air becomes charged with germs through constant sweeping and dusting, and
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