Journal of experimental zoology . ns of material killed in picro-acetic acid, sublimate-acetic,Flemmings fluid and Zenkers fluid; sections from each kind ofmaterial were stained in the following ways: (i) Iron haematoxylinfollowed by orange G; (2) thionin and orange G; (3) neutralgentian (see Bensley 00). So that at least tw^elve differentcombinations were used, and all gave essentially similar results indifferentiating the structures described. Thus it will not be neces- Elementary Phenomena of Embryonic Development 163 sary to describe the results of particular methods in detail. Mostof the
Journal of experimental zoology . ns of material killed in picro-acetic acid, sublimate-acetic,Flemmings fluid and Zenkers fluid; sections from each kind ofmaterial were stained in the following ways: (i) Iron haematoxylinfollowed by orange G; (2) thionin and orange G; (3) neutralgentian (see Bensley 00). So that at least tw^elve differentcombinations were used, and all gave essentially similar results indifferentiating the structures described. Thus it will not be neces- Elementary Phenomena of Embryonic Development 163 sary to describe the results of particular methods in detail. Mostof the features described were seen in the living egg and demon-strated also by experimental means. The cytoplasm and germinal vesicle will be described separately. I. Cytoplasm.—The main structural feature of the cytoplasmis its differentiation into two layers, an outer or ectoplasmic and aninner or endoplasmic. The ectoplasm (Fig. i) covers the free hemisphere and ends ashort distance below the equator so that the endoplasm comes to. •?-•.••;??•.-? •? ,^%\
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectzoology, bookyear1904
