. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOCYANIN-O, BINDING AND SUBUNIT COMPOSITION IN LOBSTERS 111 o in O* 0 * •* j 7 S f • • 8 o» * A ° * 0 i i i o 7i | O g 5 - 0 •o. e • 4 0 •• •• 3 ~ °o • 2 i l l I l i -. 1 .0 log free [CaCI ] o o o 8 log free [NaCI] or [Na2SOj Figure 5. Inorganic ion sensitivities of lobster hemocyanins. The units of free ion concentrations (antilogs) are mmol l~'. Left panels: H amcricanus (closed circles, solid lines). //. gammarus (open circles, dashes) and their
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOCYANIN-O, BINDING AND SUBUNIT COMPOSITION IN LOBSTERS 111 o in O* 0 * •* j 7 S f • • 8 o» * A ° * 0 i i i o 7i | O g 5 - 0 •o. e • 4 0 •• •• 3 ~ °o • 2 i l l I l i -. 1 .0 log free [CaCI ] o o o 8 log free [NaCI] or [Na2SOj Figure 5. Inorganic ion sensitivities of lobster hemocyanins. The units of free ion concentrations (antilogs) are mmol l~'. Left panels: H amcricanus (closed circles, solid lines). //. gammarus (open circles, dashes) and their hybrids (triangles, dots). mol 1~' Tns maleate buffer (pH ) + mol T1 NaCI. Right panels: the response of H americanus He to NaCI (closed circles) and Na:SO4 (open circles). mol 1' Tris maleate + mol 1~' CaCl:. 25°C. Ongin of matenal as in Figure 2. to examine specificity. The response differed very little from that of NaCI (Fig. 5), and the apparent difference may lie within the error of preparing an accurate solution of a highly hydrated salt (especially at a marine labora- tory). Discussion The essentially non-specific sensitivity of Homarus americanus He to NaCI is further evidence that the in- organic ion responses of the crustacean Hcs are not all alike. The response of this He differs from that of portunid crab Hcs (Truchot. 1975; Mason el al, 1983), which are insensitive to NaCI, but resembles that of penaid shrimp Hcs (Brouwer et 1978; Mangum and Burnett. 1985). From a physiological point of view, however, NaCI sen- sitivity is unlikely to be important in H. americanus, a stenohaline species. According to Hedgecock et ill. (1977 and pers. comm.), the genetic distance between the two parent species of Homarus. though significant, is so small that the numer- ical value is closer to expectation for subspecies than spe- cies. Thus it is of particular interest that the present find- ings support the inference of species spe
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
