. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 254 K. M. WILBUR AND A. M. BERNHARDT £ 1. ADDITION OF Cd+ + 2. INDUCTION PERIOD 3. CRYSTALLIZATION. 0 10 15 20 25 30 35 40 MINUTES FIGURE 1. Chart recording of an actual control curve showing the 3 phases of in vitro CaCO3 crystallization in artificial inorganic extrapallial fluid. Induction time (t) is indicated by the intersection of the 2 lines drawn along phases 2 and 3 on the graph. a 9 X 20 mm DEAE-Sephadex column. Preliminary experiments were undertaken to determine the appropriate pH of the bu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 254 K. M. WILBUR AND A. M. BERNHARDT £ 1. ADDITION OF Cd+ + 2. INDUCTION PERIOD 3. CRYSTALLIZATION. 0 10 15 20 25 30 35 40 MINUTES FIGURE 1. Chart recording of an actual control curve showing the 3 phases of in vitro CaCO3 crystallization in artificial inorganic extrapallial fluid. Induction time (t) is indicated by the intersection of the 2 lines drawn along phases 2 and 3 on the graph. a 9 X 20 mm DEAE-Sephadex column. Preliminary experiments were undertaken to determine the appropriate pH of the buffer and resin. A pH of was selected and 2 columns were prepared with resin allowed to swell in AIEF, pH , for 2 days. The columns were then poured and washed with 50 volumes of AIEF and rewashed with 20 volumes Tris buffer at the same pH. Measurement of the induction time of EPF, (200 ^1 EPF per ml of M Tris buffer), was carried out. A ml sample of this dilution was then applied to each of 2 columns and a ml aliquot was collected from each. This material was assayed against a control column-run sample which contained buffer alone. The columns were then eluted with ml of M NaCl in Tris buffer, and a second elution was made using M NaCl in MTris buffer. Samples of unbound and eluted materials were assayed in duplicate. Magnesium Experiments The effect of Mg on the induction time of crystallization was measured in mag- nesium-free AIEF to which MgCli was added to give final concentrations of mM Amino acid experiments Seven free and 5 polyamino acids (Sigma Chemicals) were studied for their effects on CaCO3 crystallization. The free amino acids included aspartic and glutamic acids, glycine, leucine, lysine, histidine, and taurine. Polyamino acids tested were polyaspartic and polyglutamic acids, polyglycine, polyleucine, polylysine, and polyhistidine. All free amino acids were tested at concentrations up to X 10"4
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
