. Aquatic surveys and assessment of the Slim Buttes region of Harding and Butte Co., SD . Figure 8. Multi-habitat macroinvertebrate sampling using EPA protocols. ethanol and brought to the MTNHP lab in Helena for processing. These samples were processed (sorting, identification, and data analysis) by David Stagliano at the Helena lab following DEQ protocols (MT DEQ 2005). Macroinvertebrates were identified to the lowest taxonomic level, imported into EDAS (Jessup 2006), and biological metrics were calculated from the data using the Montana Department of Environmental Quality's newest multimet
. Aquatic surveys and assessment of the Slim Buttes region of Harding and Butte Co., SD . Figure 8. Multi-habitat macroinvertebrate sampling using EPA protocols. ethanol and brought to the MTNHP lab in Helena for processing. These samples were processed (sorting, identification, and data analysis) by David Stagliano at the Helena lab following DEQ protocols (MT DEQ 2005). Macroinvertebrates were identified to the lowest taxonomic level, imported into EDAS (Jessup 2006), and biological metrics were calculated from the data using the Montana Department of Environmental Quality's newest multimetric macroinvertebrate (MMI) protocols (Jessup et al. 2005, Feldman 2006). Metric results were then scored using the Montana DEQ bioassessment criteria and each sample categorized as non-impaired or impaired according to threshold values (Table 3). The macroinvertebrate MMI score is based upon a series of metrics that measure attributes of benthic macroinvertebrate communities regarding condition changes to a stream system (in the form of pollution or pollutants). The invertebrate metrics include: EPT Taxa Richness (Score = EPT richness/14* 100): Ephemeroptera, Plecoptera &Trichoptera taxa; Percent Tanypodinae (Score = PercentTanypodinae/10 *100): Percent Orthocladiinae of Chironomidae (Score = (100-percent Orthocladiinae of Chironomidae/ 100)*100); Predator Taxa Richness (Score = number of predator taxa/9*100); Percent Collectors and Filterers (Score = (100 - percent collectors and filterers/65)*100): This metric measures the relative abundance of collector and filterer taxa in the sample. The index score represents the condition of the macroinvertebrate community at the time the sample was collected within that past year. If the index score is below the impairment threshold, the individual metrics can be used to provide insight as to why the communities are different from the reference condition (Barbour et al. 1999, Jessup et al. 2005). The impairment threshold set by MT
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