. A text-book of bacteriology. Bacteriology. 480 BACILLI IN CHRONIC INFECTIOUS DISEASES. uated pipette; in spreading these uniformly by means of a platinum needle and a turn table; in covering the dried film with a film of blood serum, and coagulating this by heat; and, finally, in staining and counting the bacilli in a series of slides from the same specimen, and from the average number found in a single drop estimating the total number in the sputum for twenty-four hours. Pathogenesis.—Man, cattle, and monkeys are most subject to contract the disease naturally, and it may be communicated by
. A text-book of bacteriology. Bacteriology. 480 BACILLI IN CHRONIC INFECTIOUS DISEASES. uated pipette; in spreading these uniformly by means of a platinum needle and a turn table; in covering the dried film with a film of blood serum, and coagulating this by heat; and, finally, in staining and counting the bacilli in a series of slides from the same specimen, and from the average number found in a single drop estimating the total number in the sputum for twenty-four hours. Pathogenesis.—Man, cattle, and monkeys are most subject to contract the disease naturally, and it may be communicated by in- oculation to many of the lower animals—guinea-pigs, field mice, rab-. FiG. 119.—Limited epithelioid celled tubercle of the iris. X 950. CBaumsarten i bits, and cats are among the most susceptible animals ; and in larger doses dogs, rats, white mice, and fowls may also be infected. When tuberculous sputum is introduced beneath the skin of a guinea-pig the nearest lymphatic glands are found to be swollen at the end of two or three weeks, at the same time there is a thickening of the tissues about the point of inoculation ; later a dry crust forms over the local tuberculous tumefaction, and beneath this is a flattened ulcer covered with cheesy material. The animals become emaciated and show difficulty in breathing, and usually succumb to general tuberculosis, especially involving the lungs, within four to eight weeks. Injections of tuberculous sputum, or of pure cultures of the bacillus, into the peritoneal cavity give rise to extensive tuberculo- sis of the liver, spleen, and lungs, and to death, as a rule, within three or four weeks. Rabbits are less susceptible to subcutaneous. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sternberg, George Miller, 1838-1915. New York, W. Wood and company
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