. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. TUBES 141 TUBES. Esmarch Tubes.—^A useful modification of the plating method just described is that suggested by von Esmarch. It insures the greatest security from contamination by extraneous organisms and requires the least amount of apparatus. It differs from the other methods thus: the dilutions having been prepared in tubes contain a smaller amount of medium than usual—as a rule, not more than 5 to 6 —are, instead of being poured upon plates or into dishes, spread over the inner surface of t
. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. TUBES 141 TUBES. Esmarch Tubes.—^A useful modification of the plating method just described is that suggested by von Esmarch. It insures the greatest security from contamination by extraneous organisms and requires the least amount of apparatus. It differs from the other methods thus: the dilutions having been prepared in tubes contain a smaller amount of medium than usual—as a rule, not more than 5 to 6 —are, instead of being poured upon plates or into dishes, spread over the inner surface of the tubes containing Fig. 26. Metal cooling stage. them, and, without removing the cotton plugs, solidified in this position. The tubes then present a thin cylindrical lining of gelatin or agar-agar, upon which the colonies develop. In all other respects the conditions for the growth of the organisms are the same as in flat plates. The solidification of the media on the inner sides of the tubes is best accomplished by rolling them upon a block of ice (Fig. 27), after the plan devised by Booker in 1887 in the Pathological Laboratory of the Johns Hopkins Univer- sity. In this method a small block of ice only is Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Abbott, Alexander Crever, 1860-1935. Philadelphia, Lea & Febiger
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