. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. i- 8 . / ; '. 9 \ :•*. v FIGURES 8 and 9. A small agar slab of growing L-Pm was placed in a drop slide (Fig. 8; ), then inoculated with 1-2 bacteria and examined under a compound light microscope. After two days (Fig. 9; l,340x), there were cavities formed in the hyphal cytoplasm. After six days (not shown), the fungal hyphae were completely devoid of cytoplasm. No stain. Antagonistic effects oj bacteria There were 177 bacterial isolates obtained from the duplicate 10 2 dilution plates from embryo samples of fifteen fe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. i- 8 . / ; '. 9 \ :•*. v FIGURES 8 and 9. A small agar slab of growing L-Pm was placed in a drop slide (Fig. 8; ), then inoculated with 1-2 bacteria and examined under a compound light microscope. After two days (Fig. 9; l,340x), there were cavities formed in the hyphal cytoplasm. After six days (not shown), the fungal hyphae were completely devoid of cytoplasm. No stain. Antagonistic effects oj bacteria There were 177 bacterial isolates obtained from the duplicate 10 2 dilution plates from embryo samples of fifteen females taken from their natural environment. Of these, 27 (15%) restricted fungal growth at least 1 mm from the edge of the bacterial colony. An additional 18 (10%) retarded fungal growth by slowing the rate or de- creasing the density of the hyphae. Two bacteria, 1-2 and 1-13, isolated from embryos on females held in the lab- oratory system, restricted growth of L-Pm on 1/3 MA plates (Fig. 5). Zones of inhibition were 6-10 mm and 4-8 mm, respectively. Growth of L-Pm from agar slabs in broth (1/3 MB) was luxuriant and easily observable after only 1 day, whereas there was no growth in broth inoculated with 1-2 or 1-13 bacteria. These bacteria are shown in Figures 6 and 7. Fungal growth across Nalgene Nutrient Pad Kit membranes, hydrated with ster- ile 1/3 SW, was thick and luxuriant, reaching a 1-2 mm radius the first day and increasing to over 4 mm by the third day. Fungal slabs retrieved from the membrane, trimmed, and plated on 1/3 MA showed 6-10 mm growth in 24 hours. No growth, however, was recorded after 3 days by fungi on nutrient pads hydrated with broth culture of 1-2 bacteria. Fungal slabs retrieved from this treatment after 1 day grew 6-8 mm when plated on 1/3 MA for 24 hours, but those retrieved after 2 days grew only 0-2 mm in 24 hours and those retrieved after 3 days did not grow even after 48 hours on 1/3 MA. Figures 8 and 9 show the degradation of L-Pm by bacter
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
