. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. X-RAYS AND CYTOPLASMIC INJURY 215 The chance that the array of ratios in section A, column 4, is a random variation of uniformity in expectation is infinitesimal according to the x~ test (P = ). Facts relevant to the discussion of cytoplasmic injury are expressed graphically in Figure 1. They fall into two categories, those related to hatchability of unferti- lized eggs x-rayed in prophase I (x-rayed chromosomes in x-rayed cytoplasm) and those related to incidence of androgenetic males (untreated chromosomes in x-ray


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. X-RAYS AND CYTOPLASMIC INJURY 215 The chance that the array of ratios in section A, column 4, is a random variation of uniformity in expectation is infinitesimal according to the x~ test (P = ). Facts relevant to the discussion of cytoplasmic injury are expressed graphically in Figure 1. They fall into two categories, those related to hatchability of unferti- lized eggs x-rayed in prophase I (x-rayed chromosomes in x-rayed cytoplasm) and those related to incidence of androgenetic males (untreated chromosomes in x-rayed cytoplasm). The 95 per cent confidence interval for each experimental value is plotted in the figure. Tables of confidence of Ricker (1937) and of Clopper and Pearson (1934) were 30 DOSE x 10 T FIGURE 2. Percentages of hatchability of unfertilized prophase I eggs plotted semilogarith- mically against dose; control and continuous treatments (•), fractionated treatments (O). By method of least squares a straight line was fitted to data of untreated, one minute (continuous + fractionated), two minutes (continuous), four minutes (fractionated) and five minutes (frac- tionated). For significance of experimental values consult Figure 1. For hatchability of eggs x-rayed in prophase I with continuous treatment, there are two dose-action curves; writh fractionated treatment, one. The former curves are not exponential, the latter is (Fig. 2). Note that the dose at which response to fractionation becomes apparent is about 15,000 r. The curve representing androgenetic males per female rises to about 15,000 r and then falls until the lethal dose for them is reached. A steadily increasing num- ber of chromatin bridges with resultant increase in retarded egg pronuclei is to be expected with increase of dose, so that androgenetic males should increase steadily with dose if there were not a concomitant increase of some factor which reduces their viability. Since this factor is x-ray


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology