. dai xie tang dan bai yu dan bai ju tang jie gou gong neng he dai xie. botany. åä½-SEPHAROSESB Vo ï¼20 1. çé ¸è½¯éª¨ç´ 'è·¯ 2. NaOH/NaBH4 30 t Vt 8 SEPHADEX G-75. å¾78ä»å¹å »çé¸¡ç¿ è½¯éª¨ç»èå离çèç½èç³ã ä¸å¾ï¼çº¯åçåä½å¨Sepharose 2Bæ±çæ´è±æ¨¡å¼ï¼ ä¸å¾ï¼æ ·åç»è½¯éª¨ç´ èæ¶åå硼氢å纳è¿åå,å¨Sephadex G-75æ±ä¸å±æãæåºå³°ç3H主è¦å¨èç³åä¸-ï¼èæ» æ±ä½ç§¯ç3H主è¦å¨ç¡«é ¸è½¯éª¨ç´ çæ¶å产ç©äºç³ä¸-ã (æèªLuca, S. De. ct al., 1980, J. Biol. Chem., 255, 6077) æ±ä¸å±æï¼å¤§çº¦"0åå ¥çå-å¨äºç¡«é ¸è§è´¨ç´ ä¸-ï¼å¾78å³°1)ãç¶èï¼åªæé常å°ç3H æ´»åä¸ç¡«é ¸è§è´¨ç´ ä¸èµ·æ´è±ï¼ç»å¤§é¨åå¨å³°IIä¸-ãç±äºå³°nç3Hæ´»åå ä¹åªå
. dai xie tang dan bai yu dan bai ju tang jie gou gong neng he dai xie. botany. åä½-SEPHAROSESB Vo ï¼20 1. çé ¸è½¯éª¨ç´ 'è·¯ 2. NaOH/NaBH4 30 t Vt 8 SEPHADEX G-75. å¾78ä»å¹å »çé¸¡ç¿ è½¯éª¨ç»èå离çèç½èç³ã ä¸å¾ï¼çº¯åçåä½å¨Sepharose 2Bæ±çæ´è±æ¨¡å¼ï¼ ä¸å¾ï¼æ ·åç»è½¯éª¨ç´ èæ¶åå硼氢å纳è¿åå,å¨Sephadex G-75æ±ä¸å±æãæåºå³°ç3H主è¦å¨èç³åä¸-ï¼èæ» æ±ä½ç§¯ç3H主è¦å¨ç¡«é ¸è½¯éª¨ç´ çæ¶å产ç©äºç³ä¸-ã (æèªLuca, S. De. ct al., 1980, J. Biol. Chem., 255, 6077) æ±ä¸å±æï¼å¤§çº¦"0åå ¥çå-å¨äºç¡«é ¸è§è´¨ç´ ä¸-ï¼å¾78å³°1)ãç¶èï¼åªæé常å°ç3H æ´»åä¸ç¡«é ¸è§è´¨ç´ ä¸èµ·æ´è±ï¼ç»å¤§é¨åå¨å³°IIä¸-ãç±äºå³°nç3Hæ´»åå ä¹åªå-å¨äºç é²ç³åºï¼æ以èç½èç³åä½ä¸-大å¤æ°ççé²ç³åºå-å¨äºä¸ç¡«é ¸è§è´¨ç´ ä¸åç寡ç³ä¸-ã ä»ç§»æ¤çSwarmå¤§é¼ è½¯éª¨ç¤å离çèç½èç³åä½çå®éªï¼é¨å解å³äºç»æåè¿äº 寡ç³çå ³ç³»ãè¿ç§è¿ç¤çèç½èç³ä¸å«ç¡«é ¸è§è´¨ç´ ï¼ä½å-å¨N-è¿æ¥çå¯äºçé²ç³çå¯¡ç³ é¨åï¼å¤§çº¦80%çN-è¿æ¥ç寡ç³å-å¨äºæ ¸å¿èç½ä¸éæè´¨é ¸ç»ååºçãåå-é为67 000 çåºåä¸-ã0-è¿æ¥ç寡ç³ä¸»è¦ä½äºç¡«é ¸è½¯éª¨ç´ è½æ®µãå®ä»¬å¯å解为3个主è¦æåï¼AãB åC),è¿ä¸ä¸ªæåçç»æï¼å¾79)ç±ç²åºåå质谱åææµå®ãä¸ç³ï¼C)ååç³ï¼B)åå« æåä¹³ç³åº1â3 N-ä¹é °åä¹³ç³èºéï¼è¿ç§ç»ææ¯ç»ç¢±æ§ç¡¼æ°¢åé å¤çåï¼å离ç大å¤æ° 0-è¿æ¥ç寡ç³çè¿æ¥åºä¸-çé常ç»æãæ大ç寡ç³ï¼A)çç»æï¼ç
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