. Bulletin of the Department of Agriculture. Agriculture. EUROPEAN FOULBROOD. 13 Glucose gelatin plate.—At refrigerator temperature aud within 3 days, the surface colonies begin to liquefy the gelatin, each liquefied area appearing somewhat as a minute drop of water. Agar slant.—In one day numerous gray colonies cover the inoculated surface. Bouillon.—Within a day the medium is uniformly and moderately clouded. Fermentation.—In glucose, lactose, saccharose, levulose, maltose, and man- nite bouillons, a uniform clouding of the media occurs. The growth takes place in both arms of the tube,
. Bulletin of the Department of Agriculture. Agriculture. EUROPEAN FOULBROOD. 13 Glucose gelatin plate.—At refrigerator temperature aud within 3 days, the surface colonies begin to liquefy the gelatin, each liquefied area appearing somewhat as a minute drop of water. Agar slant.—In one day numerous gray colonies cover the inoculated surface. Bouillon.—Within a day the medium is uniformly and moderately clouded. Fermentation.—In glucose, lactose, saccharose, levulose, maltose, and man- nite bouillons, a uniform clouding of the media occurs. The growth takes place in both arms of the tube, but is heavier in the open one. Considerable acidity, but no gas, is produced. Milk.—Milk is rapidly coagulated. Digestion of the coagulum follows. In from 3 to 5 days more than one-half has been changed. Within 24 hours the color is discharged in litmus milk, except at the top of the medium. In other respects it is like the plain milk. Potato.—No visible growth. That growth in the potato water takes place is confirmed by microscopic examination. Gelatin stab.—Liquefaction along the line of puncture is appreciable after one day. In four days a cylinder of liquefied gelatin 1 cm. in diameter surrounds the original line of puncture and soon extends to the walls of the tube. Pathogenesis.—No disease results when the brood of bees is fed cultures of Streptococcus apis either by the direct or indirect method. A rabbit and two guinea pigs inoculated with a pure cul- ture of Streptococcus apis were not sus- ceptible to infection with the species. BACTERIUM EURYDICE The presence of this species in European foulbrood was pointed out by the writer in an earlier pub- lication (15). Among the second- ary invaders in larvae infected with Bacillus plwton, Bacterium eury- ^'«- 4^--S'^<^^rium euryMce. dice is one of the earliest to be found. It is often present in consid- erable numbers. In plating for the species the stomach contents from larvse sick, but not dead, of the
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