. The microscope; an introduction to microscopic methods and to histology. Microscopes. Ch. XT ISOLATION OF TISSUE ELEMENTS 33T^ that the cells and fibers may be separated from one another, and at the same time the cells are preserved. In fixing and hardening, on the other hand, the cell cement, hke the other parts of the tissue, is made firmer. In preparing the isolating solutions it is better to. Fig. 196, 197. Shellvial and Comstock, Bent-neck Specimen Bottle. Fig. 196. Shellvial with turned lip. One can have almost any size and length desired. Those of 22 x 65 nun. and 30 x 90 mm. have bee
. The microscope; an introduction to microscopic methods and to histology. Microscopes. Ch. XT ISOLATION OF TISSUE ELEMENTS 33T^ that the cells and fibers may be separated from one another, and at the same time the cells are preserved. In fixing and hardening, on the other hand, the cell cement, hke the other parts of the tissue, is made firmer. In preparing the isolating solutions it is better to. Fig. 196, 197. Shellvial and Comstock, Bent-neck Specimen Bottle. Fig. 196. Shellvial with turned lip. One can have almost any size and length desired. Those of 22 x 65 nun. and 30 x 90 mm. have been found most useful. The larger ones are excellent for staining single slides or pairs. Fig. 197. The Comstock, bent-neck specimen bottle is very useful for keeping small animals straight. dilute the fixing agents with normal salt solution than merely with water (§ 584).' § 515. Example of isolation. — Place a piece of the trachea of a very recently killed animal, or the roof of a frog's mouth, in formalde- hyde dissociator in a shell vial or glass box. After half an hour, up to two or three days, excellent preparations of ciUated cells may be obtained by scraping the trachea or roof of the mouth and mounting the scrapings on a slide. If one proceeds after one hour, probably most of the cells will cHng together, and in the various clumps will appear cells on end showing the cilia or the bases of the cells, and other clumps will show the cells in profile. By tapping the cover. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Gage, Simon Henry, 1851-1944. [Ithaca, N. Y. The Comstock publishing company
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