Dental cosmos . ibers appear aswhite dots in the center of the black-stained Neumanns sheath. The HISTOGENY AND HISTOLOGY OF BONY AND DENTAL TISSUES. I 279 nearer to the pulp the section is taken, the more surely will the doublestaining succeed. I saw it most perfectly in the undeveloped teeth ofMacropus lugens (marsupial). If the section is taken through theperipheral ends of the dentinal tubules, then the double staining will notsucceed, especially in old teeth, because the balsam does not penetratedeeply enough into the thin Tomess fibers. The combined staining after v. Kochs and Golgis met
Dental cosmos . ibers appear aswhite dots in the center of the black-stained Neumanns sheath. The HISTOGENY AND HISTOLOGY OF BONY AND DENTAL TISSUES. I 279 nearer to the pulp the section is taken, the more surely will the doublestaining succeed. I saw it most perfectly in the undeveloped teeth ofMacropus lugens (marsupial). If the section is taken through theperipheral ends of the dentinal tubules, then the double staining will notsucceed, especially in old teeth, because the balsam does not penetratedeeply enough into the thin Tomess fibers. The combined staining after v. Kochs and Golgis methods hasgiven me an entirely new clue as to the true nature of the very delicateconnecting branches between the dentinal tubules. All former authorshave regarded them to be lateral protoplasmic branches of Tomessfibers. Waldeyer and others believed that there were connectingbridges between the odontoblasts, and that these became the finelateral branches. For a long time I tried, but in vain, to find the Fig. Fig. 16.—Longitudinal ground section through the crown of a cuspid of man (twenty-threeyears) Wet ground section, stained with Golgis method. J, interglobular spaces ; S, diam. supposed connecting bridges between the odontoblasts. Once, whenexamining the young odontoblasts of a cat, I thought I saw such aconnection in the neighborhood of the dentine end of those cells,but then found that I had been deceived by the oblique turning offof Tomess processes. If the supposed connections were reallypresent, then they ought to be present also in older teeth, and alsoin isolated preparations. But no one has succeeded in finding themthere. V. v. Ebner and v. Kolliker therefore supposed that theconnecting branches of the dentinal tubules arise secondarily by asprouting out of Tomess fibers. This explanation seems to me stillmore improbable, as nowhere in the animal kingdom has such a phe-nomenon been observed as a process of a connective-tissue cell givingoff numerou
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