. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 206 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS A 100 — 80 § 40 O" 0) U_ 20 • C ontrol cells D ME.'!-treated':-His n. n. 0 4 U 5 0 6 07 08 09 Ratio Lshort. Figure 1. Cells treated with MBC exhihil a misplaced nucleus and septum. Fission yeast cells expressing nucleoporin-GFP ami GFP-lnhidin were imaged at room temperature (21"-23"C) with time-lapse fluorescence microscopy. lAI Pint ot distribution f'ret/iieney of nuclear positions ex- pressed as the ratio £.1/l,,r(/L;,,,li;. where /,,,„„, is the length Ir
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 206 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS A 100 — 80 § 40 O" 0) U_ 20 • C ontrol cells D ME.'!-treated':-His n. n. 0 4 U 5 0 6 07 08 09 Ratio Lshort. Figure 1. Cells treated with MBC exhihil a misplaced nucleus and septum. Fission yeast cells expressing nucleoporin-GFP ami GFP-lnhidin were imaged at room temperature (21"-23"C) with time-lapse fluorescence microscopy. lAI Pint ot distribution f'ret/iieney of nuclear positions ex- pressed as the ratio £.1/l,,r(/L;,,,li;. where /,,,„„, is the length Irom the center of the nucleus to the short cell tip and /.,„„,. is tin- length from the center of the nucleus to t/ie opposite long cell lip. (B) Wild-type cells through one cell cvclc- Lett panels arc I>IC images s/un\ ing septum formation at the middle of the icll I at 0 h and later ul 4 hi. Right panels arc fluorescent images of nuclear membrane and microtubules. Microtubules span the length of the cell during interphase. As the cell grew, nuclei were posi- tioned h\ Jsnnnn, mi, roinhnles at the center of the cells where siihsci/ucnt cell division and scptanon o, i iirred. creating two daughter cells of ap- pro\inui!cl\' cifiuil length. \i>t< ihe disappearance / interphase nncrotu- hulcs in the cell cytoplasm iind the appearance «/ the nntotic spindle inside the cell nucleus during nnlosis i at 4 hi. tCl MBC-treated cells through one cell c\cle. No microtubules weic present in MBC-treated cells. \Vithoui position of the plane of cell division and the septum. Cells which have lost their microtubule cytoskeleton can continue to grow, and can undergo septation and cytokinesis after a cell cycle delay. Our results are consistent with phenotypes seen in studies of tuhulin mutants (7). However, our experimental conditions ensure an almost complete loss of the microtubule cytoskeleton; and our time-lapse microscopy allows long-term viewing of the develop- men
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
