. Botany; an elementary text for schools. Plants. 244 THE CELL small sliding microtome and a sharp razor are necessary. Cut one of the pieces of collodion into an oblong block with the imbedded material near one end. This can be clamped in the microtome, be- ing held in place by a flat piece of cork on either side. The collodion must project above the cork. The razjr should be adjusted in such a manner that the wiiole length of the blade is used in cutting. The blade should be tilted downwards so tha*-, only the cutting edge comes in contact with the block which should not be scraped by the lo
. Botany; an elementary text for schools. Plants. 244 THE CELL small sliding microtome and a sharp razor are necessary. Cut one of the pieces of collodion into an oblong block with the imbedded material near one end. This can be clamped in the microtome, be- ing held in place by a flat piece of cork on either side. The collodion must project above the cork. The razjr should be adjusted in such a manner that the wiiole length of the blade is used in cutting. The blade should be tilted downwards so tha*-, only the cutting edge comes in contact with the block which should not be scraped by the lower flat surface of the razor back of the edge. Both the collodion block and the razor must be kept flooded with alcohol during the process of cutting. When several sections have been cut they may be flouted out on a slide and arranged near the center. Then with a pipette place a drop of ether on the sections. This partially dis- solves the collodion and thus sticks the sections to the slide. The slide is then covered with water to remove the alcohol, after which it is ready for staining. Sections are ruined if allowed to become dry at any time after cutting. 4. Stain with hematoxylin for from three to five minutes, and wash off the surplus stain with water. 5. Drain off the water and dehydrate by keeping the slide flooded witn alcohol for ten minutes, or by placing it in a vessel of alcohol. 6. Pour off the alcohol and cover the slide with a clearing mix- ture (see p. 241) and allow it to stand for ten minutes. The clearer removes the alcohol which cannot mix with balsam. 7. Drain and wipe off as much of the clearer as possible with- out touching the sections. Then place a small drop of prepared Canada balsam on the sections near the center of the slide, and with a pair of forceps lay on a clean cover-glass. If the proper amount of balsam has been used it will spread out to the edge of the cover- glass without exuding. The slide is now ready to be examined. It should be cleane
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