. The Biological bulletin. Biology; Zoology; Marine biology. METABOLISM OF SULFUR AMINO ACIDS 177. o CA CSA T Figure 2. Strip chromatogram (lower) and radioactivity distribution (upper) from the acid fractions of R. cuneata. Solvent: phenol-water. Cysteic acid (CA), cysteine sulfmic acid (CSA) and taurine (T). intermediates were recognized by their Rf values (cystathionine in 72% phenol and in 65% lutidine; hypotaurine in 72% phenol and in 65% lutidine). Spot 1 is methionine and spot 2, probably methionine sulfone. 2) Formation of cystine Fraction B, from part 1, was used t
. The Biological bulletin. Biology; Zoology; Marine biology. METABOLISM OF SULFUR AMINO ACIDS 177. o CA CSA T Figure 2. Strip chromatogram (lower) and radioactivity distribution (upper) from the acid fractions of R. cuneata. Solvent: phenol-water. Cysteic acid (CA), cysteine sulfmic acid (CSA) and taurine (T). intermediates were recognized by their Rf values (cystathionine in 72% phenol and in 65% lutidine; hypotaurine in 72% phenol and in 65% lutidine). Spot 1 is methionine and spot 2, probably methionine sulfone. 2) Formation of cystine Fraction B, from part 1, was used to identify cystine or cysteine. Neither of these amino acids can be chromatographed satisfactorily. Cystine is very insoluble and lends itself to isolation. In both instances unlabelled cystine hydrochloride was added to the fractions. Then hydrogen peroxide was added to oxidize cysteine to cystine. Cystine was precipitated from the solution by carefully adjusting the pH to 7. The crystals were filtered, washed, redissolved in dilute HC1 and O CA T Figure 3. Strip chromatogram and radioactivity distribution from the acid fraction of M. cdulis. Cysteic acid (CA) and taurine (T).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report; HighWire Press. Lancaster, Pa. [etc. ] : Lancaster Press, inc. [etc. ]
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